TY - JOUR
T1 - Phytochemical analysis, antioxidant, antifungal activity, and genotoxicity of essential oil and solvent extracts of tetraclinis articulata leaves from northern Morocco
AU - Benoutman, Amina
AU - Erbiai, El Hadi
AU - Ettakifi, Hajar
AU - Legssyer, Mounir
AU - Handaq, Nadia
AU - Makrane, Hanane
AU - Saidi, Rabah
AU - Lamrani, Zouhaire
AU - Pintado, Manuela
AU - Pinto, Eugénia
AU - Silva, Joaquim C. G. Esteves da
AU - Maouni, Abdelfettah
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/3/30
Y1 - 2024/3/30
N2 - Tetraclinis articulata is a medicinal plant commonly known in Morocco as” Araar Tree “. Various parts of T. articulata are traditionally used for various therapeutic purposes. In this context, the present study aims to evaluate the antioxidant, antifungal, and genotoxicity of methanolic (TaLME), acetonic (TaLAE) extracts, and essential oil (TaLEO) from Tetraclinis articulata leaves growing in northern Morocco. This was carried out through chemical analysis, bioactive substance determination, and essential oil and extract testing. The bioactive substances determined by the spectrophotometric method showed a higher content of phenols and flavonoids in TaLME. The Liquid chromatography-mass spectrometry (LC-MS) analysis of phenolic compounds revealed protocatechuic acid as predominant in TaLME, followed by paraben acid and syringic acid, and in TaLAE, p-hydroxybenzoic was the principal compound, followed by protocatechuic acid and vanillic acid. The gas chromatography-mass spectrometry (GC-MS) analysis of TaLEO (yield of 0.77 %) revealed the presence of 22 compounds (95.80 %) with main components (%) α-pinene (29.62), bornyl acetate (21.45), and camphor (16.80). Biological activities were evaluated: antioxidant efficiency using four different methods (DPPH, ABTS, FRAP, ORAC), deoxyribonucleic acid (DNA) protection, genotoxicity test, and antifungal activity against five pathogenic fungi (Aspergillus fumigatus, Candida albicans, Epidermophyton floccosum, Trichophyton rubrum, and Microsporum canis). According to the results, the extracts and EO had significant antioxidant and antifungal activity against the dermatophytes. The genotoxicity test exhibited that the T. articulata extracts and EO were bereft of any mutagenic activity. Overall, this study demonstrates that Tetraclinis articulata plant essential oil and extracts can represent an intriguing potential source of natural compounds that can be helpful for future investigations.
AB - Tetraclinis articulata is a medicinal plant commonly known in Morocco as” Araar Tree “. Various parts of T. articulata are traditionally used for various therapeutic purposes. In this context, the present study aims to evaluate the antioxidant, antifungal, and genotoxicity of methanolic (TaLME), acetonic (TaLAE) extracts, and essential oil (TaLEO) from Tetraclinis articulata leaves growing in northern Morocco. This was carried out through chemical analysis, bioactive substance determination, and essential oil and extract testing. The bioactive substances determined by the spectrophotometric method showed a higher content of phenols and flavonoids in TaLME. The Liquid chromatography-mass spectrometry (LC-MS) analysis of phenolic compounds revealed protocatechuic acid as predominant in TaLME, followed by paraben acid and syringic acid, and in TaLAE, p-hydroxybenzoic was the principal compound, followed by protocatechuic acid and vanillic acid. The gas chromatography-mass spectrometry (GC-MS) analysis of TaLEO (yield of 0.77 %) revealed the presence of 22 compounds (95.80 %) with main components (%) α-pinene (29.62), bornyl acetate (21.45), and camphor (16.80). Biological activities were evaluated: antioxidant efficiency using four different methods (DPPH, ABTS, FRAP, ORAC), deoxyribonucleic acid (DNA) protection, genotoxicity test, and antifungal activity against five pathogenic fungi (Aspergillus fumigatus, Candida albicans, Epidermophyton floccosum, Trichophyton rubrum, and Microsporum canis). According to the results, the extracts and EO had significant antioxidant and antifungal activity against the dermatophytes. The genotoxicity test exhibited that the T. articulata extracts and EO were bereft of any mutagenic activity. Overall, this study demonstrates that Tetraclinis articulata plant essential oil and extracts can represent an intriguing potential source of natural compounds that can be helpful for future investigations.
KW - Antifungal activity
KW - Antioxidant activity
KW - Biochemical composition
KW - Essential oil
KW - Flavonoids
KW - Genotoxicity test
KW - Phenols
KW - Tetraclinis articulata
UR - http://www.scopus.com/inward/record.url?scp=85205691942&partnerID=8YFLogxK
U2 - 10.33263/LIANBS131.050
DO - 10.33263/LIANBS131.050
M3 - Article
AN - SCOPUS:85205691942
SN - 2284-6808
VL - 13
JO - Letters in Applied NanoBioScience
JF - Letters in Applied NanoBioScience
IS - 1
M1 - 50
ER -