Plant aqueous extracts: antioxidant capacity via haemolysis and bacteriophage P22 protection

Maria S. Gião; Isabel Leitão; Ana Pereira; André B. Borges; Catarina J. Guedes; João C. Fernandes; Luís Belo; Alice Santos-Silva; Tim A. Hogg; Manuela E. Pintado; F. Xavier Malcata

doi:10.1016/j.foodcont.2009.08.014

Plant aqueous extracts: antioxidant capacity via haemolysis and bacteriophage P22 protection

Maria S. Gião, Isabel Leitão, Ana Pereira, André B. Borges, Catarina J. Guedes, João C. Fernandes, Luís Belo, Alice Santos-Silva, Tim A. Hogg, Manuela E. Pintado, F. Xavier Malcata^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

5 Downloads

Abstract

The bacteriophage P22/Salmonella Typhimurium system, as well as human erythrocytes have been used to assay for protection, against forced oxidation caused by hydrogen peroxide, brought about by several aqueous extracts of selected adventitious plants grown in Portugal. This study proved, for the first time, that the aforementioned bacteriophage-based system is a suitable method to assess the antioxidant activity of plant extracts; among the 12 plants tested, raspberry (Rubus idaeus), sage (Salvia sp.), savory (Satureja montana) and yarrow (Achillea millefolium) were found to effectively protect against oxidative damage caused by H₂O₂. Haemolysis was inhibited via pre-treatment with every plant extract tested, except heath at 0.1% (w/v). The two analytical methods produced different results - and for some plants, there was a dependence (either direct or inverse) of the quantitative protection effect on extract concentration, whereas for others no significant dependence was found at all. Savory yielded the most promising results, using either method. Therefore, the P22/Salmonella system can be used as a suitable in vivo assay, and human erythrocytes as a suitable in vitro assay to confirm (or not) the antioxidant capacity of plant extracts in biological matrices.

Original language	English
Pages (from-to)	633-638
Number of pages	6
Journal	Food Control
Volume	21
Issue number	5
DOIs	https://doi.org/10.1016/j.foodcont.2009.08.014
Publication status	Published - May 2010

Keywords

Erythrocytes
In vivo assays
Medicinal plants
Salmonella Typhimurium

Access to Document

10.1016/j.foodcont.2009.08.014

http://hdl.handle.net/10400.14/2656

Cite this

@article{b9a2fb754f3b43b59d6f7a2d98b51225,

title = "Plant aqueous extracts: antioxidant capacity via haemolysis and bacteriophage P22 protection",

abstract = "The bacteriophage P22/Salmonella Typhimurium system, as well as human erythrocytes have been used to assay for protection, against forced oxidation caused by hydrogen peroxide, brought about by several aqueous extracts of selected adventitious plants grown in Portugal. This study proved, for the first time, that the aforementioned bacteriophage-based system is a suitable method to assess the antioxidant activity of plant extracts; among the 12 plants tested, raspberry (Rubus idaeus), sage (Salvia sp.), savory (Satureja montana) and yarrow (Achillea millefolium) were found to effectively protect against oxidative damage caused by H2O2. Haemolysis was inhibited via pre-treatment with every plant extract tested, except heath at 0.1% (w/v). The two analytical methods produced different results - and for some plants, there was a dependence (either direct or inverse) of the quantitative protection effect on extract concentration, whereas for others no significant dependence was found at all. Savory yielded the most promising results, using either method. Therefore, the P22/Salmonella system can be used as a suitable in vivo assay, and human erythrocytes as a suitable in vitro assay to confirm (or not) the antioxidant capacity of plant extracts in biological matrices.",

keywords = "Erythrocytes, In vivo assays, Medicinal plants, Salmonella Typhimurium",

author = "Gi{\~a}o, {Maria S.} and Isabel Leit{\~a}o and Ana Pereira and Borges, {Andr{\'e} B.} and Guedes, {Catarina J.} and Fernandes, {Jo{\~a}o C.} and Lu{\'i}s Belo and Alice Santos-Silva and Hogg, {Tim A.} and Pintado, {Manuela E.} and Malcata, {F. Xavier}",

year = "2010",

month = may,

doi = "10.1016/j.foodcont.2009.08.014",

language = "English",

volume = "21",

pages = "633--638",

journal = "Food Control",

issn = "0956-7135",

publisher = "Elsevier B.V.",

number = "5",

}

TY - JOUR

T1 - Plant aqueous extracts

T2 - antioxidant capacity via haemolysis and bacteriophage P22 protection

AU - Gião, Maria S.

AU - Leitão, Isabel

AU - Pereira, Ana

AU - Borges, André B.

AU - Guedes, Catarina J.

AU - Fernandes, João C.

AU - Belo, Luís

AU - Santos-Silva, Alice

AU - Hogg, Tim A.

AU - Pintado, Manuela E.

AU - Malcata, F. Xavier

PY - 2010/5

Y1 - 2010/5

N2 - The bacteriophage P22/Salmonella Typhimurium system, as well as human erythrocytes have been used to assay for protection, against forced oxidation caused by hydrogen peroxide, brought about by several aqueous extracts of selected adventitious plants grown in Portugal. This study proved, for the first time, that the aforementioned bacteriophage-based system is a suitable method to assess the antioxidant activity of plant extracts; among the 12 plants tested, raspberry (Rubus idaeus), sage (Salvia sp.), savory (Satureja montana) and yarrow (Achillea millefolium) were found to effectively protect against oxidative damage caused by H2O2. Haemolysis was inhibited via pre-treatment with every plant extract tested, except heath at 0.1% (w/v). The two analytical methods produced different results - and for some plants, there was a dependence (either direct or inverse) of the quantitative protection effect on extract concentration, whereas for others no significant dependence was found at all. Savory yielded the most promising results, using either method. Therefore, the P22/Salmonella system can be used as a suitable in vivo assay, and human erythrocytes as a suitable in vitro assay to confirm (or not) the antioxidant capacity of plant extracts in biological matrices.

AB - The bacteriophage P22/Salmonella Typhimurium system, as well as human erythrocytes have been used to assay for protection, against forced oxidation caused by hydrogen peroxide, brought about by several aqueous extracts of selected adventitious plants grown in Portugal. This study proved, for the first time, that the aforementioned bacteriophage-based system is a suitable method to assess the antioxidant activity of plant extracts; among the 12 plants tested, raspberry (Rubus idaeus), sage (Salvia sp.), savory (Satureja montana) and yarrow (Achillea millefolium) were found to effectively protect against oxidative damage caused by H2O2. Haemolysis was inhibited via pre-treatment with every plant extract tested, except heath at 0.1% (w/v). The two analytical methods produced different results - and for some plants, there was a dependence (either direct or inverse) of the quantitative protection effect on extract concentration, whereas for others no significant dependence was found at all. Savory yielded the most promising results, using either method. Therefore, the P22/Salmonella system can be used as a suitable in vivo assay, and human erythrocytes as a suitable in vitro assay to confirm (or not) the antioxidant capacity of plant extracts in biological matrices.

KW - Erythrocytes

KW - In vivo assays

KW - Medicinal plants

KW - Salmonella Typhimurium

UR - http://www.scopus.com/inward/record.url?scp=73549116726&partnerID=8YFLogxK

U2 - 10.1016/j.foodcont.2009.08.014

DO - 10.1016/j.foodcont.2009.08.014

M3 - Article

AN - SCOPUS:73549116726

SN - 0956-7135

VL - 21

SP - 633

EP - 638

JO - Food Control

JF - Food Control

IS - 5

ER -

Plant aqueous extracts: antioxidant capacity via haemolysis and bacteriophage P22 protection

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this