Plant extract with bioactive potential against SARS-CoV-2

Adma N. F. Melo; Tiago B. Afonso; Marta Carvalho; Cláudia Rodrigues; Tânia Ribeiro; Márcio  Carocho; Miguel Marques Pinto; Freni Tavaria; Paula Teixeira; J. Pedro Simas; Lillian Barros; Manuela Pintado

Abstract

Background: The novel disease caused by SARS-CoV-2 has been causing chaos worldwide due to its high mortality, morbidity and contagiousness nature, and therefore forced the scientific world to develop new alternatives to combat and assist the fight against this virus. Plant extracts have bioactive molecules that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents able to inhibit or neutralize COVID-19. Therefore, the objective of this study was to obtain an extract from a Plant cultivated under controlled conditions in Portugal to be used as an antiviral substance to be applied against COVID-19. Materials/Methods: The plant extract obtained from plant cultivated under controlled conditions in Portugal was extracted by maceration in a hydroethanolic solution under optimized conditions and submitted to freeze drying. The extracts were evaluated for their phenolic profile by high performance liquid chromatography coupled to a diode array detector and a mass spectrometer detector (HPLC-DAD-MS). Antioxidant activity was also measured, and antiviral properties were measured for SARS-CoV-2 in Vero cells. Results. HPLC analysis revealed the presence of different phenolic compounds in the extracts with relevance for caftaric acid, cis-chicoric acid and trans-chychoric acid. The extract showed a good antioxidant capacity demonstrated by the high value found for ORAC of 3202,82 ± 32,52 Trolox/mg extract. Regarding the antiviral activity, the results achieved for the viral titer was 20 PFU/mL, and the antiviral activity for SARS-CoV-2 was 4.02 ± 0.00 Mv and the percentage of reduction was > 99.99%. Conclusion: The results showed that the obtained extract demonstrated consistent results of antiviral activity presenting a potential for applications against the SARS-CoV-2. Further studies are required for validation and application of this extract.

Original language	English
Pages	1-1
Number of pages	1
Publication status	Published - 2021
Event	4th International Congress of Biochemistry & Microbiology Applied Technologies - Hammamet, Tunisia Duration: 5 Nov 2021 → 7 Nov 2021

Conference

Conference	4th International Congress of Biochemistry & Microbiology Applied Technologies
Abbreviated title	BMAT-2021
Country/Territory	Tunisia
City	Hammamet
Period	5/11/21 → 7/11/21

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

Melo, A. N. F., Afonso, T. B., Carvalho, M., Rodrigues, C., Ribeiro, T., Carocho, M., Pinto, M. M., Tavaria, F., Teixeira, P., Simas, J. P., Barros, L., & Pintado, M. (2021). Plant extract with bioactive potential against SARS-CoV-2. 1-1. Abstract from 4th International Congress of Biochemistry & Microbiology Applied Technologies, Hammamet, Tunisia. http://hdl.handle.net/10400.14/41066

@conference{c63e94f292954757ae510e278de9ddaf,

title = "Plant extract with bioactive potential against SARS-CoV-2",

abstract = "Background: The novel disease caused by SARS-CoV-2 has been causing chaos worldwide due to its high mortality, morbidity and contagiousness nature, and therefore forced the scientific world to develop new alternatives to combat and assist the fight against this virus. Plant extracts have bioactive molecules that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents able to inhibit or neutralize COVID-19. Therefore, the objective of this study was to obtain an extract from a Plant cultivated under controlled conditions in Portugal to be used as an antiviral substance to be applied against COVID-19. Materials/Methods: The plant extract obtained from plant cultivated under controlled conditions in Portugal was extracted by maceration in a hydroethanolic solution under optimized conditions and submitted to freeze drying. The extracts were evaluated for their phenolic profile by high performance liquid chromatography coupled to a diode array detector and a mass spectrometer detector (HPLC-DAD-MS). Antioxidant activity was also measured, and antiviral properties were measured for SARS-CoV-2 in Vero cells. Results. HPLC analysis revealed the presence of different phenolic compounds in the extracts with relevance for caftaric acid, cis-chicoric acid and trans-chychoric acid. The extract showed a good antioxidant capacity demonstrated by the high value found for ORAC of 3202,82 ± 32,52 Trolox/mg extract. Regarding the antiviral activity, the results achieved for the viral titer was 20 PFU/mL, and the antiviral activity for SARS-CoV-2 was 4.02 ± 0.00 Mv and the percentage of reduction was > 99.99\%. Conclusion: The results showed that the obtained extract demonstrated consistent results of antiviral activity presenting a potential for applications against the SARS-CoV-2. Further studies are required for validation and application of this extract. ",

author = "Melo, \{Adma N. F.\} and Afonso, \{Tiago B.\} and Marta Carvalho and Cl{\'a}udia Rodrigues and T{\^a}nia Ribeiro and M{\'a}rcio Carocho and Pinto, \{Miguel Marques\} and Freni Tavaria and Paula Teixeira and Simas, \{J. Pedro\} and Lillian Barros and Manuela Pintado",

year = "2021",

language = "English",

pages = "1--1",

note = "4th International Congress of Biochemistry \& Microbiology Applied Technologies, BMAT-2021 ; Conference date: 05-11-2021 Through 07-11-2021",

}

Melo, ANF , Afonso, TB , Carvalho, M, Rodrigues, C, Ribeiro, T, Carocho, M, Pinto, MM, Tavaria, F , Teixeira, P , Simas, JP, Barros, L & Pintado, M 2021, 'Plant extract with bioactive potential against SARS-CoV-2', 4th International Congress of Biochemistry & Microbiology Applied Technologies, Hammamet, Tunisia, 5/11/21 - 7/11/21 pp. 1-1. <http://hdl.handle.net/10400.14/41066>

TY - CONF

T1 - Plant extract with bioactive potential against SARS-CoV-2

AU - Melo, Adma N. F.

AU - Afonso, Tiago B.

AU - Carvalho, Marta

AU - Rodrigues, Cláudia

AU - Ribeiro, Tânia

AU - Carocho, Márcio

AU - Pinto, Miguel Marques

AU - Tavaria, Freni

AU - Teixeira, Paula

AU - Simas, J. Pedro

AU - Barros, Lillian

AU - Pintado, Manuela

PY - 2021

Y1 - 2021

N2 - Background: The novel disease caused by SARS-CoV-2 has been causing chaos worldwide due to its high mortality, morbidity and contagiousness nature, and therefore forced the scientific world to develop new alternatives to combat and assist the fight against this virus. Plant extracts have bioactive molecules that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents able to inhibit or neutralize COVID-19. Therefore, the objective of this study was to obtain an extract from a Plant cultivated under controlled conditions in Portugal to be used as an antiviral substance to be applied against COVID-19. Materials/Methods: The plant extract obtained from plant cultivated under controlled conditions in Portugal was extracted by maceration in a hydroethanolic solution under optimized conditions and submitted to freeze drying. The extracts were evaluated for their phenolic profile by high performance liquid chromatography coupled to a diode array detector and a mass spectrometer detector (HPLC-DAD-MS). Antioxidant activity was also measured, and antiviral properties were measured for SARS-CoV-2 in Vero cells. Results. HPLC analysis revealed the presence of different phenolic compounds in the extracts with relevance for caftaric acid, cis-chicoric acid and trans-chychoric acid. The extract showed a good antioxidant capacity demonstrated by the high value found for ORAC of 3202,82 ± 32,52 Trolox/mg extract. Regarding the antiviral activity, the results achieved for the viral titer was 20 PFU/mL, and the antiviral activity for SARS-CoV-2 was 4.02 ± 0.00 Mv and the percentage of reduction was > 99.99%. Conclusion: The results showed that the obtained extract demonstrated consistent results of antiviral activity presenting a potential for applications against the SARS-CoV-2. Further studies are required for validation and application of this extract.

AB - Background: The novel disease caused by SARS-CoV-2 has been causing chaos worldwide due to its high mortality, morbidity and contagiousness nature, and therefore forced the scientific world to develop new alternatives to combat and assist the fight against this virus. Plant extracts have bioactive molecules that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents able to inhibit or neutralize COVID-19. Therefore, the objective of this study was to obtain an extract from a Plant cultivated under controlled conditions in Portugal to be used as an antiviral substance to be applied against COVID-19. Materials/Methods: The plant extract obtained from plant cultivated under controlled conditions in Portugal was extracted by maceration in a hydroethanolic solution under optimized conditions and submitted to freeze drying. The extracts were evaluated for their phenolic profile by high performance liquid chromatography coupled to a diode array detector and a mass spectrometer detector (HPLC-DAD-MS). Antioxidant activity was also measured, and antiviral properties were measured for SARS-CoV-2 in Vero cells. Results. HPLC analysis revealed the presence of different phenolic compounds in the extracts with relevance for caftaric acid, cis-chicoric acid and trans-chychoric acid. The extract showed a good antioxidant capacity demonstrated by the high value found for ORAC of 3202,82 ± 32,52 Trolox/mg extract. Regarding the antiviral activity, the results achieved for the viral titer was 20 PFU/mL, and the antiviral activity for SARS-CoV-2 was 4.02 ± 0.00 Mv and the percentage of reduction was > 99.99%. Conclusion: The results showed that the obtained extract demonstrated consistent results of antiviral activity presenting a potential for applications against the SARS-CoV-2. Further studies are required for validation and application of this extract.

M3 - Abstract

SP - 1

EP - 1

T2 - 4th International Congress of Biochemistry & Microbiology Applied Technologies

Y2 - 5 November 2021 through 7 November 2021

ER -