Platform design for extraction and isolation of Bromelain: complex formation and precipitation with carrageenan

Débora A. Campos, Nadia Woitovich Valetti, Ana Oliveira, Lorenzo M. Pastrana-Castro, José A. Teixeira, Maria Manuela Pintado*, Guillermo Picó

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
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Abstract

The main objective of this work was to investigate for the first time the molecular mechanism of complex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strong polyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. The Bromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility was dependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH was changed and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism for the formation of non-soluble complex. The formation of the carrageenan-bromelain complex increased in 8 °C the enzyme thermal stability, while its biological activity was not modified. The amount of total enzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85–90%.

Original languageEnglish
Pages (from-to)156-161
Number of pages6
JournalProcess Biochemistry
Volume54
DOIs
Publication statusPublished - Mar 2017

Keywords

  • Bromelain
  • Carrageenan
  • Enzyme mechanism
  • Polyelectrolytes
  • Protein complex

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