Abstract
Aim: This work aims to investigate the adjuvant effect of poly-ϵ-caprolactone/chitosan nanoparticles (NPs) for hepatitis B surface antigen (HBsAg) and the plasmid DNA encoding HBsAg (pRC/CMV-HBs). Methods: Both antigens were adsorbed onto preformed NPs. Vaccination studies were performed in C57BL/6 mice. Transfection efficiency was investigated in A549 cell line. Results: HBsAg-adsorbed NPs generated strong anti-HBsAg IgG titers, mainly of IgG1 isotype, and induced antigen-specific IFN-γ and IL-17 secretion by spleen cells. The addition of pRC/CMV-HBs to the HBsAg-adsorbed NPs inhibited IL-17 secretion but had minor effect on IFN-γ levels. Lastly, pRC/CMV-HBs-loaded NPs generated a weak serum antibody response. Conclusion: Poly-ϵ-caprolactone/chitosan NPs provide a strong humoral adjuvant effect for HBsAg and induce a Th1/Th17-mediated cellular immune responses worth explore for hepatitis B virus vaccination.
| Original language | English |
|---|---|
| Pages (from-to) | 2335-2348 |
| Number of pages | 14 |
| Journal | Nanomedicine |
| Volume | 12 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - Oct 2017 |
| Externally published | Yes |
Keywords
- HBsAg
- Hepatitis B
- pDNA vaccine
- Poly-ϵ-caprolactone (PCL)/chitosan nanoparticles
- Vaccine adjuvants
