Polyelectrolytes–protein complexes: a viable platform in the downstream processes of industrial enzymes at scaling up level

Nadia Woitovich Valetti, M. Emilia Brassesco, Guillermo Alfredo Picó*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

19 Citations (Scopus)

Abstract

Complexation between proteins and polyelectrolytes through electrostatic interactions gives either soluble or non-soluble complexes, leading to phase separation. The formation and the stability of these complexes are influenced by pH, ionic strength, ratio between protein and polyelectrolyte and charge density of protein and polyelectrolyte. This review presented the advantages on the protein precipitation by adding a polyelectrolyte solution to a natural enzyme source (microbiological, vegetal or animal homogenate), over the classical precipitation methods. An enzyme can be precipitated like non-soluble complex, separated and then re-dissolved, thus the method works, at the same time, like concentrative and pre-purification steps together.
Original languageEnglish
Pages (from-to)2921-2928
Number of pages8
JournalJournal of Chemical Technology and Biotechnology
Volume91
Issue number12
DOIs
Publication statusPublished - 1 Dec 2016
Externally publishedYes

Keywords

  • Downstream processes, bioseparation
  • Enzyme purification
  • Polyelectrolytes

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