TY - JOUR
T1 - Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform
T2 - effect of the surfactants on characteristics and delivery efficiency
AU - Rebanda, Magda M.
AU - Bettini, Simona
AU - Blasi, Laura
AU - Gaballo, Antonio
AU - Ragusa, Andrea
AU - Quarta, Alessandra
AU - Piccirillo, Clara
N1 - Funding Information:
Acknowledgments: The authors are grateful to Luca Bizzarro and Alessandra Cavallo for the experimental support. The authors would like to acknowledge the support of the following Italian projects: “Tecnopolo per la medicina di precisione” (TecnoMed Puglia-Regione Puglia: DGR n.2117 del 21 November 2018, CUP: B84I18000540002, “Tecnopolo di Nanotecnologia e Fotonica per la medicina di precisione” (TECNOMED)-FISR/MIUR-CNR: delibera CIPE n.3449 del 7 August 2017, CUP: B83B17000010001 and SEARCULAR, “Estrazione di composti bioattivi da scarti dell’industria ittica e loro valorizzazione in ambito cosmetico”.
Funding Information:
The authors are grateful to Luca Bizzarro and Alessandra Cavallo for the experimental support. The authors would like to acknowledge the support of the following Italian projects: ?Tecnopolo per la medicina di precisione? (TecnoMed Puglia-Regione Puglia: DGR n.2117 del 21 November 2018, CUP: B84I18000540002, ?Tecnopolo di Nanotecnologia e Fotonica per la medicina di precisione? (TECNOMED)-FISR/MIUR-CNR: delibera CIPE n.3449 del 7 August 2017, CUP: B83B17000010001 and SEARCULAR, ?Estrazione di composti bioattivi da scarti dell?industria ittica e loro valorizzazione in ambito cosmetico?.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5/1
Y1 - 2022/5/1
N2 - Polymeric nanoparticles made of the copolymer Poly(L-lactide-co-caprolactone-co-glycolide) were prepared using the solvent evaporation method. Two different surfactants, polyvinyl alcohol and dextran, and a mixture of the two were employed. The three types of nanoparticles were used as hosting carriers of two chemotherapeutic drugs, the hydrophilic doxorubicin and the hydrophobic SN-38. The morphostructural characterization showed similar features for the three types of nanoparticles, while the drug encapsulation efficiency indicated that the dextran-based systems are the most effective with both drugs. Cellular studies with breast cancer cells were performed to compare the delivery capability and the cytotoxicity profile of the three nanosystems. The results show that the unloaded nanoparticles are highly biocompatible at the administered concentrations and confirmed that dextran-coated nanoparticles are the most efficient vectors to release the two drugs, exerting cytotoxic activity. PVA, on the other hand, shows limited drug release in vitro, probably due to strong interactions with both drugs. Data also show the release is more efficient for doxorubicin than for SN-38; indeed, the doxorubicin IC50 value for the dextran-coated nanoparticles was about 35% lower than the free drug. This indicates that these nanocarriers are suitable candidates to deliver hydrophilic drugs while needing further modification to host hydrophobic molecules.
AB - Polymeric nanoparticles made of the copolymer Poly(L-lactide-co-caprolactone-co-glycolide) were prepared using the solvent evaporation method. Two different surfactants, polyvinyl alcohol and dextran, and a mixture of the two were employed. The three types of nanoparticles were used as hosting carriers of two chemotherapeutic drugs, the hydrophilic doxorubicin and the hydrophobic SN-38. The morphostructural characterization showed similar features for the three types of nanoparticles, while the drug encapsulation efficiency indicated that the dextran-based systems are the most effective with both drugs. Cellular studies with breast cancer cells were performed to compare the delivery capability and the cytotoxicity profile of the three nanosystems. The results show that the unloaded nanoparticles are highly biocompatible at the administered concentrations and confirmed that dextran-coated nanoparticles are the most efficient vectors to release the two drugs, exerting cytotoxic activity. PVA, on the other hand, shows limited drug release in vitro, probably due to strong interactions with both drugs. Data also show the release is more efficient for doxorubicin than for SN-38; indeed, the doxorubicin IC50 value for the dextran-coated nanoparticles was about 35% lower than the free drug. This indicates that these nanocarriers are suitable candidates to deliver hydrophilic drugs while needing further modification to host hydrophobic molecules.
KW - Doxorubicin
KW - Polymeric nanoparticle
KW - SN-38
KW - Surfactant-drug interaction
UR - http://www.scopus.com/inward/record.url?scp=85129125792&partnerID=8YFLogxK
U2 - 10.3390/nano12091550
DO - 10.3390/nano12091550
M3 - Article
C2 - 35564258
AN - SCOPUS:85129125792
SN - 2079-4991
VL - 12
JO - Nanomaterials
JF - Nanomaterials
IS - 9
M1 - 1550
ER -
