TY - JOUR
T1 - Potential biological properties of lycopene in a self-emulsifying drug delivery system
AU - Báo, Sônia Nair
AU - Machado, Manuela
AU - Silva, Ana Luisa da
AU - Melo, Adma
AU - Cunha, Sara
AU - Sousa, Sérgio S.
AU - Malheiro, Ana Rita
AU - Fernandes, Rui
AU - Leite, José Roberto S. A.
AU - Vasconcelos, Andreanne G.
AU - Relvas, João
AU - Pintado, Manuela
N1 - Funding Information:
This research was funded by the National Council for Scientific and Technological Development—CNPq (303557/2021-4); the Coordination for the Improvement of Higher-Level Personnel—CAPES (0001); the Financier of Studies and Projects—FINEP (01.08.0457.00); the Federal District Research Support Foundation—FAPDF (23106.128959/2021-69); and supported in part by the Coordination for the Improvement of Higher-Level Personnel—CAPES project number 1713871 and co-funded by People&Science Pesquisa, Desenvolvimento e Inovação Ltd.a ME, Brazil. This work was also supported by National Funds from FCT—Fundação para a Ciência e a Tecnologia through project UIDB/50016/2020.
Funding Information:
The authors acknowledge CNPq, CAPES, FINEP, and FAPDF for the funding of this project.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/2
Y1 - 2023/2
N2 - In recent years, lycopene has been highlighted due to its antioxidant and anti-inflammatory properties, associated with a beneficial effect on human health. The aim of this study was to advance the studies of antioxidant and anti-inflammatory mechanisms on human keratinocytes cells (HaCaT) of a self-emulsifying drug delivery system (SEDDS) loaded with lycopene purified from red guava (nanoLPG). The characteristics of nanoLPG were a hydrodynamic diameter of 205 nm, a polydispersity index of 0.21 and a zeta potential of −20.57, providing physical stability for the nanosystem. NanoLPG demonstrated antioxidant capacity, as shown using the ORAC methodology, and prevented DNA degradation (DNA agarose). Proinflammatory activity was evaluated by quantifying the cytokines TNF-α, IL-6 and IL-8, with only IL-8 showing a significant increase (p < 0.0001). NanoLPG showed greater inhibition of the tyrosinase and elastase enzymes, involved in the skin aging process, compared to purified lycopene (LPG). In vitro treatment for 24 h with 5.0 µg/mL of nanoLPG did not affect the viability of HaCaT cells. The ultrastructure of HaCaT cells demonstrated the maintenance of morphology. This contrasts with endoplasmic reticulum stresses and autophagic vacuoles when treated with LPG after stimulation or not with LPS. Therefore, the use of lycopene in a nanoemulsion may be beneficial in strategies and products associated with skin health.
AB - In recent years, lycopene has been highlighted due to its antioxidant and anti-inflammatory properties, associated with a beneficial effect on human health. The aim of this study was to advance the studies of antioxidant and anti-inflammatory mechanisms on human keratinocytes cells (HaCaT) of a self-emulsifying drug delivery system (SEDDS) loaded with lycopene purified from red guava (nanoLPG). The characteristics of nanoLPG were a hydrodynamic diameter of 205 nm, a polydispersity index of 0.21 and a zeta potential of −20.57, providing physical stability for the nanosystem. NanoLPG demonstrated antioxidant capacity, as shown using the ORAC methodology, and prevented DNA degradation (DNA agarose). Proinflammatory activity was evaluated by quantifying the cytokines TNF-α, IL-6 and IL-8, with only IL-8 showing a significant increase (p < 0.0001). NanoLPG showed greater inhibition of the tyrosinase and elastase enzymes, involved in the skin aging process, compared to purified lycopene (LPG). In vitro treatment for 24 h with 5.0 µg/mL of nanoLPG did not affect the viability of HaCaT cells. The ultrastructure of HaCaT cells demonstrated the maintenance of morphology. This contrasts with endoplasmic reticulum stresses and autophagic vacuoles when treated with LPG after stimulation or not with LPS. Therefore, the use of lycopene in a nanoemulsion may be beneficial in strategies and products associated with skin health.
KW - Antioxidant
KW - Carotenoid
KW - Guava fruit
KW - Nanomedicine
KW - Skin care
UR - http://www.scopus.com/inward/record.url?scp=85147956897&partnerID=8YFLogxK
U2 - 10.3390/molecules28031219
DO - 10.3390/molecules28031219
M3 - Article
C2 - 36770886
SN - 1420-3049
VL - 28
JO - Molecules
JF - Molecules
IS - 3
M1 - 1219
ER -