Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications

P. Coimbra; P. Ferreira; H. C. de Sousa; P. Batista; M. A. Rodrigues; I. J. Correia; M. H. Gil

doi:10.1016/j.ijbiomac.2010.10.006

Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications

P. Coimbra^*, P. Ferreira, H. C. de Sousa, P. Batista, M. A. Rodrigues, I. J. Correia, M. H. Gil

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

184 Citations (Scopus)

Abstract

In this work, porous scaffolds obtained from the freeze-drying of pectin/chitosan polyelectrolyte complexes were prepared and characterized by FTIR, SEM and weight loss studies. Additionally, the cytotoxicity of the prepared scaffolds was evaluated in vitro, using human osteoblast cells. The results obtained showed that cells adhered to scaffolds and proliferated. The study also confirmed that the degradation by-products of pectin/chitosan scaffold are noncytotoxic.

Original language	English
Pages (from-to)	112-118
Number of pages	7
Journal	International Journal of Biological Macromolecules
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.ijbiomac.2010.10.006
Publication status	Published - 1 Jan 2011
Externally published	Yes

Keywords

Cytotoxicity
Osteoblasts
Polyelectrolyte complexes
Polymeric scaffold

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10.1016/j.ijbiomac.2010.10.006

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Coimbra, P., Ferreira, P., de Sousa, H. C., Batista, P., Rodrigues, M. A., Correia, I. J., & Gil, M. H. (2011). Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications. International Journal of Biological Macromolecules, 48(1), 112-118. https://doi.org/10.1016/j.ijbiomac.2010.10.006

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title = "Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications",

abstract = "In this work, porous scaffolds obtained from the freeze-drying of pectin/chitosan polyelectrolyte complexes were prepared and characterized by FTIR, SEM and weight loss studies. Additionally, the cytotoxicity of the prepared scaffolds was evaluated in vitro, using human osteoblast cells. The results obtained showed that cells adhered to scaffolds and proliferated. The study also confirmed that the degradation by-products of pectin/chitosan scaffold are noncytotoxic.",

keywords = "Cytotoxicity, Osteoblasts, Polyelectrolyte complexes, Polymeric scaffold",

author = "P. Coimbra and P. Ferreira and {de Sousa}, {H. C.} and P. Batista and Rodrigues, {M. A.} and Correia, {I. J.} and Gil, {M. H.}",

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Coimbra, P, Ferreira, P, de Sousa, HC, Batista, P, Rodrigues, MA, Correia, IJ & Gil, MH 2011, 'Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications', International Journal of Biological Macromolecules, vol. 48, no. 1, pp. 112-118. https://doi.org/10.1016/j.ijbiomac.2010.10.006

Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications. / Coimbra, P.; Ferreira, P.; de Sousa, H. C. et al.
In: International Journal of Biological Macromolecules, Vol. 48, No. 1, 01.01.2011, p. 112-118.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications

AU - Coimbra, P.

AU - Ferreira, P.

AU - de Sousa, H. C.

AU - Batista, P.

AU - Rodrigues, M. A.

AU - Correia, I. J.

AU - Gil, M. H.

PY - 2011/1/1

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N2 - In this work, porous scaffolds obtained from the freeze-drying of pectin/chitosan polyelectrolyte complexes were prepared and characterized by FTIR, SEM and weight loss studies. Additionally, the cytotoxicity of the prepared scaffolds was evaluated in vitro, using human osteoblast cells. The results obtained showed that cells adhered to scaffolds and proliferated. The study also confirmed that the degradation by-products of pectin/chitosan scaffold are noncytotoxic.

AB - In this work, porous scaffolds obtained from the freeze-drying of pectin/chitosan polyelectrolyte complexes were prepared and characterized by FTIR, SEM and weight loss studies. Additionally, the cytotoxicity of the prepared scaffolds was evaluated in vitro, using human osteoblast cells. The results obtained showed that cells adhered to scaffolds and proliferated. The study also confirmed that the degradation by-products of pectin/chitosan scaffold are noncytotoxic.

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KW - Osteoblasts

KW - Polyelectrolyte complexes

KW - Polymeric scaffold

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JF - International Journal of Biological Macromolecules

IS - 1

ER -

Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications

Abstract

Keywords

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