TY - JOUR
T1 - Production of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinamer
AU - Costa, A. da
AU - Pereira, A. M.
AU - Gomes, A. C.
AU - Rodriguez-Cabello, J. C.
AU - Casal, M.
AU - Machado, R.
N1 - Funding Information:
This work was supported by national funds through the FCT I.P. and by the ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) [strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569)]. The present work was also supported by FCT within the ERA-NET IB, project FunBioPlas [grant number ERA-IB-15-089 and FCT reference ERA-IB-2-6/0004/2014]. AMPereira work was funded by DP_AEM and FCT [grant PD/BD/113811/2015]. This article is a result of the project EcoAgriFood [NORTE-01-0145-FEDER-000009], supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).
Funding Information:
This work was supported by national funds through the FCT I.P. and by the ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) [strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569)]. The present work was also supported by FCT within the ERA-NET IB, project FunBioPlas [grant number ERA-IB-15-089 and FCT reference ERA-IB-2-6/0004/2014 ]. AMPereira work was funded by DP_AEM and FCT [grant PD/BD/113811/2015 ]. This article is a result of the project EcoAgriFood [NORTE-01-0145-FEDER-000009], supported by Norte Portugal Regional Operational Programme (NORTE 2020) , under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/11/25
Y1 - 2018/11/25
N2 - With the lack of new chemical antibiotics and increasing pathogen resistance to those available, new alternatives are being explored. Antimicrobial peptides (AMPs) with a broad range of effects, including antibacterial, antifungal, and antiviral actions, have emerged as one of the options. They can be produced by recombinant DNA technology, but the chromatographic methods used for peptide purification are expensive and time consuming. Here, we describe the design, production, purification and assessment of the antibacterial activity of the human peptide hepcidin, using an elastin-like recombinamer as fusion partner. The recombinant protein Hep-A200 was produced in Escherichia coli and purified by a non-chromatographic procedure, exploiting the thermal properties of the A200 elastin-like recombinamer. Recombinant Hep-A200 was found to retain antibacterial activity against Gram-positive and Gram-negative species.
AB - With the lack of new chemical antibiotics and increasing pathogen resistance to those available, new alternatives are being explored. Antimicrobial peptides (AMPs) with a broad range of effects, including antibacterial, antifungal, and antiviral actions, have emerged as one of the options. They can be produced by recombinant DNA technology, but the chromatographic methods used for peptide purification are expensive and time consuming. Here, we describe the design, production, purification and assessment of the antibacterial activity of the human peptide hepcidin, using an elastin-like recombinamer as fusion partner. The recombinant protein Hep-A200 was produced in Escherichia coli and purified by a non-chromatographic procedure, exploiting the thermal properties of the A200 elastin-like recombinamer. Recombinant Hep-A200 was found to retain antibacterial activity against Gram-positive and Gram-negative species.
KW - Antimicrobial activity
KW - Antimicrobial peptide
KW - Elastin-Like recombinamer
KW - Hepcidin
UR - http://www.scopus.com/inward/record.url?scp=85050360823&partnerID=8YFLogxK
U2 - 10.1016/j.nbt.2018.07.001
DO - 10.1016/j.nbt.2018.07.001
M3 - Article
C2 - 30012422
AN - SCOPUS:85050360823
SN - 1871-6784
VL - 46
SP - 45
EP - 53
JO - New Biotechnology
JF - New Biotechnology
ER -