TY - JOUR
T1 - Production of marine probiotic bacteria in a cost-effective marine media based on peptones obtained from discarded fish by-products
AU - Vázquez, José Antonio
AU - Durán, Ana
AU - Nogueira, Margarita
AU - Menduíña, Araceli
AU - Antunes, Joana
AU - Freitas, Ana Cristina
AU - Gomes, Ana María
N1 - Funding Information:
Funding: This research was funded by the projects CVMar+i (0302_CVMAR_I_1_P, POCTEP 2015) and Xunta de Galicia (Grupos de Potencial Crecimiento, IN607B 2018/19). This work was also supported by national funds through FCT/MEC (PIDDAC), project reference IF/00588/2015.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/8
Y1 - 2020/8
N2 - The industrial production of marine bacteria with probiotic properties is limited by the excessive cost of the culture media adequate for their growth. The present work aimed to study the suitability of 30 marine media formulated with nitrogen sources (fish peptones) from different fish discards and seawater, for the growth of two marine probiotic bacteria (MPB), namely Phaeobacter sp. and Pseudomonas fluorescens. These fish peptones were produced from several discarded fish and by-products (heads, skins, and whole individuals of megrim, mackerel, gurnard, hake, etc.). In all cultivations, no significant differences were found on cell viability when the microorganism grew on commercial or alternative media. In relation to the biomass production, the growth of Phaeobacter sp. on waste media was commonly similar or a 20% lower than observed in the control cultures. For P. fluorescens, various peptones (skin peptones of pouting and blue whiting) showed even higher productive ability than commercial peptones. An initial economical evaluation revealed that low-cost media reduced until 120 times the cost of production of MPB.
AB - The industrial production of marine bacteria with probiotic properties is limited by the excessive cost of the culture media adequate for their growth. The present work aimed to study the suitability of 30 marine media formulated with nitrogen sources (fish peptones) from different fish discards and seawater, for the growth of two marine probiotic bacteria (MPB), namely Phaeobacter sp. and Pseudomonas fluorescens. These fish peptones were produced from several discarded fish and by-products (heads, skins, and whole individuals of megrim, mackerel, gurnard, hake, etc.). In all cultivations, no significant differences were found on cell viability when the microorganism grew on commercial or alternative media. In relation to the biomass production, the growth of Phaeobacter sp. on waste media was commonly similar or a 20% lower than observed in the control cultures. For P. fluorescens, various peptones (skin peptones of pouting and blue whiting) showed even higher productive ability than commercial peptones. An initial economical evaluation revealed that low-cost media reduced until 120 times the cost of production of MPB.
KW - Fish discards valorization
KW - Fish peptones
KW - Logistic equation
KW - Low-cost marine media
KW - Marine probiotics bacteria production
KW - Sustainability
UR - http://www.scopus.com/inward/record.url?scp=85090627186&partnerID=8YFLogxK
U2 - 10.3390/microorganisms8081121
DO - 10.3390/microorganisms8081121
M3 - Article
C2 - 32722528
AN - SCOPUS:85090627186
SN - 2076-2607
VL - 8
SP - 1
EP - 15
JO - Microorganisms
JF - Microorganisms
IS - 8
M1 - 1121
ER -
