TY - JOUR
T1 - Projection structures reveal the position of the DNA within DNA-Dps Co-crystals
AU - Moiseenko, Andrey
AU - Loiko, Natalia
AU - Tereshkina, Ksenia
AU - Danilova, Yana
AU - Kovalenko, Vladislav
AU - Chertkov, Oleg
AU - Feofanov, Alexey V.
AU - Krupyanskii, Yurii F.
AU - Sokolova, Olga S.
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/9/24
Y1 - 2019/9/24
N2 - One of the universal mechanisms for the response of Escherichia coli to stress is the increase of the synthesis of specific histone-like proteins that bind the DNA, Dps. As a result, two-and three-dimensional crystalline arrays may be observed in the cytoplasm of starving cells. Here, we determined the conditions to obtain very thin two-dimensional DNA-Dps co-crystals in vitro, and studied their projection structures, using electron microscopy. Analysis of the projection maps of the free Dps crystals revealed two lattice types: hexagonal and rectangular. We used the fluorescently labeled DNA to prove that the DNA is present within the co-crystals with Dps in vitro, and visualized its position using transmission electron microscopy. Molecular modeling confirmed the DNA position within the crystal. We have also suggested a structural model for the DNA-Dps co-crystal dissolving in the presence of Mg2+ ions.
AB - One of the universal mechanisms for the response of Escherichia coli to stress is the increase of the synthesis of specific histone-like proteins that bind the DNA, Dps. As a result, two-and three-dimensional crystalline arrays may be observed in the cytoplasm of starving cells. Here, we determined the conditions to obtain very thin two-dimensional DNA-Dps co-crystals in vitro, and studied their projection structures, using electron microscopy. Analysis of the projection maps of the free Dps crystals revealed two lattice types: hexagonal and rectangular. We used the fluorescently labeled DNA to prove that the DNA is present within the co-crystals with Dps in vitro, and visualized its position using transmission electron microscopy. Molecular modeling confirmed the DNA position within the crystal. We have also suggested a structural model for the DNA-Dps co-crystal dissolving in the presence of Mg2+ ions.
KW - Dps
KW - Electron microscopy
KW - Escherichia coli
KW - Fluorescent microscopy
KW - Fourier transform
KW - Two-dimensional crystals
UR - http://www.scopus.com/inward/record.url?scp=85069963670&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2019.07.103
DO - 10.1016/j.bbrc.2019.07.103
M3 - Article
C2 - 31376942
SN - 0006-291X
VL - 517
SP - 463
EP - 469
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -