TY - GEN
T1 - Protein and DNA nanoparticulate multiantigenic vaccines against H. pylori
T2 - 2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
AU - Figueiredo, Lara
AU - Calado, Cecilia C. R.
AU - Almeida, António J.
AU - Gonçalves, Lídia M. D.
PY - 2012
Y1 - 2012
N2 - Immunisation against H. pylori is an attractive option for antibiotic resistance and reinfection situations. Strain genetic heterogeneity, and low immunogenicity of protein antigens and DNA alone are nonetheless obstacles to this approach. We developed multigenic H. pylori DNA-nanoparticle and protein-nanoparticle vaccines based on pathogenic relevance. Six antigens were chosen for the vaccine construction: CagA, VacA, HpaA, UreB, HomB and GroEL. Different combinations of CS/DS and CS/Alg /TPP nanoparticles with DNA and chimeric proteins were produced as vaccine systems. Immune responses were evaluated after i.m. and oral immunisation of BALB/c mice. Oral vaccination successfully stimulated mucosal immunity while i.m. immunisation efficiently elicited a more equilibrated cellular/humoral immune response.
AB - Immunisation against H. pylori is an attractive option for antibiotic resistance and reinfection situations. Strain genetic heterogeneity, and low immunogenicity of protein antigens and DNA alone are nonetheless obstacles to this approach. We developed multigenic H. pylori DNA-nanoparticle and protein-nanoparticle vaccines based on pathogenic relevance. Six antigens were chosen for the vaccine construction: CagA, VacA, HpaA, UreB, HomB and GroEL. Different combinations of CS/DS and CS/Alg /TPP nanoparticles with DNA and chimeric proteins were produced as vaccine systems. Immune responses were evaluated after i.m. and oral immunisation of BALB/c mice. Oral vaccination successfully stimulated mucosal immunity while i.m. immunisation efficiently elicited a more equilibrated cellular/humoral immune response.
KW - Chitosan
KW - Helicobacter pylori
KW - Nanoparticles
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=84869454317&partnerID=8YFLogxK
U2 - 10.1109/ENBENG.2012.6331366
DO - 10.1109/ENBENG.2012.6331366
M3 - Conference contribution
AN - SCOPUS:84869454317
SN - 9781467345248
T3 - 2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
BT - 2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
Y2 - 23 February 2012 through 25 February 2012
ER -
