Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels

Joana Silva; Ana Sofia Carvalho; Rui Vitorino; Pedro Domingues; Paula Teixeira; Paul Gibbs

doi:10.2174/0929866043478293

Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels

Joana Silva, Ana Sofia Carvalho, Rui Vitorino, Pedro Domingues, Paula Teixeira^*, Paul Gibbs

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

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Abstract

Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.

Original language	English
Pages (from-to)	189-194
Number of pages	6
Journal	Protein and Peptide Letters
Volume	11
Issue number	2
DOIs	https://doi.org/10.2174/0929866043478293
Publication status	Published - Apr 2004

Keywords

2-DE electrophoresis
Lactobacillus bulgaricus
Protein extraction
Sensitivity
Staining

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http://hdl.handle.net/10400.14/6688

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AU - Gibbs, Paul

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KW - 2-DE electrophoresis

KW - Lactobacillus bulgaricus

KW - Protein extraction

KW - Sensitivity

KW - Staining

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Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels

Abstract

Keywords

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