Protocol for antioxidant dietary fiber determination: structural characterization and quantification

Tânia Bragança Ribeiro; María Emilia Brassesco; Diva Santos; Manuela Pintado

doi:10.1007/978-1-0716-3303-8_6

Protocol for antioxidant dietary fiber determination: structural characterization and quantification

Tânia Bragança Ribeiro, María Emilia Brassesco, Diva Santos, Manuela Pintado^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Identifying the dietary fiber’s (DF) chemical composition (i.e., monosaccharides, lignin, uronic acids, phenolics) is essential for a better knowledge of the various health properties of the fiber. Thus, we propose a new approach that allows us not only to quantify the content of insoluble DF (IDF) and soluble DF (SDF) but also to estimate their chemical constituents, including their phenolic compounds and their respective antioxidant activity. The method can determine the chemical structure of DF resulting from four principal steps with chemical, enzymatic, and analytical procedures. The protocol can be completed in ~10 days, including ~5 days required for the fiber profile determination.

Original language	English
Title of host publication	Food waste conversion
Editors	Cristobal N. Aguilar Gonzalez, Ricardo Gómez-García, Mohammed Kuddus
Place of Publication	New York
Publisher	Humana Press
Chapter	6
Pages	85-95
Number of pages	11
ISBN (Electronic)	9781071633038
ISBN (Print)	9781071633021, 9781071633052
DOIs	https://doi.org/10.1007/978-1-0716-3303-8_6
Publication status	Published - 23 Jul 2023

Publication series

Name	Methods and Protocols in Food Science
ISSN (Print)	2662-950X
ISSN (Electronic)	2662-9518

Keywords

Antioxidant dietary fiber
Structural carbohydrates
Bound phenolic compounds

Access to Document

10.1007/978-1-0716-3303-8_6

Cite this

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title = "Protocol for antioxidant dietary fiber determination: structural characterization and quantification",

abstract = "Identifying the dietary fiber{\textquoteright}s (DF) chemical composition (i.e., monosaccharides, lignin, uronic acids, phenolics) is essential for a better knowledge of the various health properties of the fiber. Thus, we propose a new approach that allows us not only to quantify the content of insoluble DF (IDF) and soluble DF (SDF) but also to estimate their chemical constituents, including their phenolic compounds and their respective antioxidant activity. The method can determine the chemical structure of DF resulting from four principal steps with chemical, enzymatic, and analytical procedures. The protocol can be completed in ~10 days, including ~5 days required for the fiber profile determination.",

keywords = "Antioxidant dietary fiber, Structural carbohydrates, Bound phenolic compounds",

author = "Ribeiro, {T{\^a}nia Bragan{\c c}a} and Brassesco, {Mar{\'i}a Emilia} and Diva Santos and Manuela Pintado",

year = "2023",

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doi = "10.1007/978-1-0716-3303-8_6",

language = "English",

isbn = "9781071633021",

series = "Methods and Protocols in Food Science",

publisher = "Humana Press",

pages = "85--95",

editor = "Gonzalez, {Cristobal N. Aguilar} and Ricardo G{\'o}mez-Garc{\'i}a and Mohammed Kuddus",

booktitle = "Food waste conversion",

address = "United States",

}

Protocol for antioxidant dietary fiber determination: structural characterization and quantification. / Ribeiro, Tânia Bragança ; Brassesco, María Emilia ; Santos, Diva et al.
Food waste conversion. ed. / Cristobal N. Aguilar Gonzalez; Ricardo Gómez-García; Mohammed Kuddus. New York: Humana Press, 2023. p. 85-95 (Methods and Protocols in Food Science).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Protocol for antioxidant dietary fiber determination

T2 - structural characterization and quantification

AU - Ribeiro, Tânia Bragança

AU - Brassesco, María Emilia

AU - Santos, Diva

AU - Pintado, Manuela

PY - 2023/7/23

Y1 - 2023/7/23

N2 - Identifying the dietary fiber’s (DF) chemical composition (i.e., monosaccharides, lignin, uronic acids, phenolics) is essential for a better knowledge of the various health properties of the fiber. Thus, we propose a new approach that allows us not only to quantify the content of insoluble DF (IDF) and soluble DF (SDF) but also to estimate their chemical constituents, including their phenolic compounds and their respective antioxidant activity. The method can determine the chemical structure of DF resulting from four principal steps with chemical, enzymatic, and analytical procedures. The protocol can be completed in ~10 days, including ~5 days required for the fiber profile determination.

AB - Identifying the dietary fiber’s (DF) chemical composition (i.e., monosaccharides, lignin, uronic acids, phenolics) is essential for a better knowledge of the various health properties of the fiber. Thus, we propose a new approach that allows us not only to quantify the content of insoluble DF (IDF) and soluble DF (SDF) but also to estimate their chemical constituents, including their phenolic compounds and their respective antioxidant activity. The method can determine the chemical structure of DF resulting from four principal steps with chemical, enzymatic, and analytical procedures. The protocol can be completed in ~10 days, including ~5 days required for the fiber profile determination.

KW - Antioxidant dietary fiber

KW - Structural carbohydrates

KW - Bound phenolic compounds

U2 - 10.1007/978-1-0716-3303-8_6

DO - 10.1007/978-1-0716-3303-8_6

M3 - Chapter

SN - 9781071633021

SN - 9781071633052

T3 - Methods and Protocols in Food Science

SP - 85

EP - 95

BT - Food waste conversion

A2 - Gonzalez, Cristobal N. Aguilar

A2 - Gómez-García, Ricardo

A2 - Kuddus, Mohammed

PB - Humana Press

CY - New York

ER -

Protocol for antioxidant dietary fiber determination: structural characterization and quantification

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Keywords

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