Protocol for the extraction of lignin from brewer’s spent grain using deep eutectic solvents

Ana C. Cassoni, Patricia Costa, Manuela Pintado*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The agro-food industry annually produces tons of lignocellulosic waste with an intrinsic high value. Specifically, lignin—an underrated bioresource that is mostly burned for energy production—has a tremendous potential as source of high-value chemicals. A promising lignin valorization requires an efficient extraction process able to recover high-purity lignin with considerable yields. Hence, we describe a protocol to extract lignin from a relevant agro-food residue—brewer’s spent grain—through the use of a deep eutectic solvent system, which has been proven an excellent potential to promote the fractionation of high-purity lignin from lignocellulosic material. The biomass is subjected to a hydrolysis at 120 °C for 5 h, and the lignin is further recovered by precipitation with distilled water. This protocol gives simple and detailed recommendations to effectively recover lignin from brewer’s spent grain contributing to the valorization of this abundant residue.
Original languageEnglish
Title of host publicationFood waste conversion
EditorsCristobal N. Aguilar Gonzalez, Ricardo Gómez-García, Mohammed Kuddus
PublisherHumana Press
Pages79-83
Number of pages5
ISBN (Electronic)9781071633038
ISBN (Print)9781071633021, 9781071633052
DOIs
Publication statusPublished - Jul 2023

Publication series

NameMethods and Protocols in Food Science
ISSN (Print)2662-950X
ISSN (Electronic)2662-9518

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  • Food waste conversion

    Gonzalez, C. N. A. (Editor), García, R. G. (Editor) & Kuddus, M. (Editor), 21 Jul 2023, New York: Humana Press. 244 p. (Methods and Protocols in Food Science)

    Research output: Book/ReportEdited bookpeer-review

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