TY - JOUR
T1 - Rat olfactory mucosa mesenchymal stem/stromal cells (OM-MSCS)
T2 - a characterization study
AU - Alvites, Rui D.
AU - Branquinho, Mariana V.
AU - Caseiro, Ana R.
AU - Amorim, Irina
AU - Pedrosa, Sílvia Santos
AU - Rêma, Alexandra
AU - Faria, Fátima
AU - Porto, Beatriz
AU - Oliveira, Cláudia
AU - Teixeira, Paula
AU - Magalhães, Rui
AU - Geuna, Stefano
AU - Varejão, Artur S. P.
AU - Maurício, Ana C.
PY - 2020
Y1 - 2020
N2 - Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.
AB - Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.
UR - http://www.scopus.com/inward/record.url?scp=85079295088&partnerID=8YFLogxK
U2 - 10.1155/2020/2938258
DO - 10.1155/2020/2938258
M3 - Article
C2 - 32411249
AN - SCOPUS:85079295088
SN - 1687-8876
VL - 2020
JO - International Journal of Cell Biology
JF - International Journal of Cell Biology
M1 - 2938258
ER -