Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy

Marta B. Lopes, Kevin C. Sales, Vitor V. Lopes, Cecilia R. C. Calado

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    1 Citation (Scopus)

    Abstract

    The development of in-situ monitoring techniques enabling the real-time acquisition of information concerning the key variables over different Escherichia coli cultivation conditions and strategies is a crucial step towards the optimization of a plasmid bioproduction process. This work shows the use of a Near-InfraRed (NIR) fiber optic probe immersed in the culture broth for the real-time acquisition of NIR spectra along different E. coli cultures conducted with mixtures of the carbon sources glucose and glycerol, and performed in both batch and fed-batch modes. Accurate partial least squares models based on the acquired spectral data over such different cultivation conditions were built, yielding a R2 = 0.97 for biomass and plasmid productions and a RMSEP of 0.34 and 7.52, respectively; a R2 of 0.93 and a RMSEP of 0.46 and 0.33 was obtained for glucose and glycerol, respectively; the acetate model produced a R2 of 0.96 and a RMSEP of 0.32.
    Original languageEnglish
    Title of host publication3rd Portuguese Bioengineering Meeting, ENBENG 2013 - Book of Proceedings
    PublisherIEEE Canada
    ISBN (Print)9781467348591
    DOIs
    Publication statusPublished - 2013
    Event2013 IEEE 3rd Portuguese Meeting in Bioengineering, ENBENG 2013 - Braga, Portugal
    Duration: 20 Feb 201322 Feb 2013

    Publication series

    Name3rd Portuguese Bioengineering Meeting, ENBENG 2013 - Book of Proceedings

    Conference

    Conference2013 IEEE 3rd Portuguese Meeting in Bioengineering, ENBENG 2013
    Country/TerritoryPortugal
    CityBraga
    Period20/02/1322/02/13

    Keywords

    • Plasmid
    • Monitoring
    • Control
    • Fourier transform infrared
    • Near-infrared
    • Spectroscopy
    • Escherichia coli

    Fingerprint

    Dive into the research topics of 'Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy'. Together they form a unique fingerprint.

    Cite this