@inproceedings{c820d420233143fd835151e464fb3b69,
title = "Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy",
abstract = "The development of in-situ monitoring techniques enabling the real-time acquisition of information concerning the key variables over different Escherichia coli cultivation conditions and strategies is a crucial step towards the optimization of a plasmid bioproduction process. This work shows the use of a Near-InfraRed (NIR) fiber optic probe immersed in the culture broth for the real-time acquisition of NIR spectra along different E. coli cultures conducted with mixtures of the carbon sources glucose and glycerol, and performed in both batch and fed-batch modes. Accurate partial least squares models based on the acquired spectral data over such different cultivation conditions were built, yielding a R2 = 0.97 for biomass and plasmid productions and a RMSEP of 0.34 and 7.52, respectively; a R2 of 0.93 and a RMSEP of 0.46 and 0.33 was obtained for glucose and glycerol, respectively; the acetate model produced a R2 of 0.96 and a RMSEP of 0.32.",
keywords = "Plasmid, Monitoring, Control, Fourier transform infrared, Near-infrared, Spectroscopy, Escherichia coli",
author = "Lopes, {Marta B.} and Sales, {Kevin C.} and Lopes, {Vitor V.} and Calado, {Cecilia R. C.}",
note = "Copyright: Copyright 2013 Elsevier B.V., All rights reserved.; 2013 IEEE 3rd Portuguese Meeting in Bioengineering, ENBENG 2013 ; Conference date: 20-02-2013 Through 22-02-2013",
year = "2013",
doi = "10.1109/ENBENG.2013.6518394",
language = "English",
isbn = "9781467348591",
series = "3rd Portuguese Bioengineering Meeting, ENBENG 2013 - Book of Proceedings",
publisher = "IEEE Canada",
booktitle = "3rd Portuguese Bioengineering Meeting, ENBENG 2013 - Book of Proceedings",
address = "Canada",
}