TY - JOUR
T1 - Recombinant CBM-fusion technology - Applications overview
AU - Oliveira, Carla
AU - Carvalho, Vera
AU - Domingues, Lucília
AU - Gama, Francisco M.
N1 - Funding Information:
C. Oliveira and V. Carvalho acknowledge support from Fundação para a Ciência e a Tecnologia (FCT), Portugal (grants SFRH/BDP/63831/2009 and SFRH/BPD/73850/2010 , respectively). The authors thank the FCT GlycoCBMs Project REF. PTDC/AGR-FOR/3090/2012 — FCOMP-01-0124-FEDER-027948, the FCT Strategic Project PEst-OE/EQB/LA0023/2013, and the Project “BioInd — Biotechnology and Bioengineering for improved Industrial and Agro-Food processes”, REF. NORTE-07-0124-FEDER-000028 Co-funded by the Programa Operacional Regional do Norte (ON.2 — O Novo Norte), QREN, FEDER.
Publisher Copyright:
© 2015 Elsevier Inc.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Carbohydrate-binding modules (CBMs) are small components of several enzymes, which present an independent fold and function, and specific carbohydrate-binding activity. Their major function is to bind the enzyme to the substrate enhancing its catalytic activity, especially in the case of insoluble substrates. The immense diversity of CBMs, together with their unique properties, has long raised their attention for many biotechnological applications. Recombinant DNA technology has been used for cloning and characterizing new CBMs. In addition, it has been employed to improve the purity and availability of many CBMs, but mainly, to construct bi-functional CBM-fused proteins for specific applications. This review presents a comprehensive summary of the uses of CBMs recombinantly produced from heterologous organisms, or by the original host, along with the latest advances. Emphasis is given particularly to the applications of recombinant CBM-fusions in: (a) modification of fibers, (b) production, purification and immobilization of recombinant proteins, (c) functionalization of biomaterials and (d) development of microarrays and probes.
AB - Carbohydrate-binding modules (CBMs) are small components of several enzymes, which present an independent fold and function, and specific carbohydrate-binding activity. Their major function is to bind the enzyme to the substrate enhancing its catalytic activity, especially in the case of insoluble substrates. The immense diversity of CBMs, together with their unique properties, has long raised their attention for many biotechnological applications. Recombinant DNA technology has been used for cloning and characterizing new CBMs. In addition, it has been employed to improve the purity and availability of many CBMs, but mainly, to construct bi-functional CBM-fused proteins for specific applications. This review presents a comprehensive summary of the uses of CBMs recombinantly produced from heterologous organisms, or by the original host, along with the latest advances. Emphasis is given particularly to the applications of recombinant CBM-fusions in: (a) modification of fibers, (b) production, purification and immobilization of recombinant proteins, (c) functionalization of biomaterials and (d) development of microarrays and probes.
KW - Carbohydrate-binding activity
KW - Carbohydrate-binding modules
KW - CBM applications
KW - Cellulose
KW - Heterologous expression systems
KW - Recombinant CBM-fusions
UR - http://www.scopus.com/inward/record.url?scp=84929511557&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2015.02.006
DO - 10.1016/j.biotechadv.2015.02.006
M3 - Review article
C2 - 25689072
AN - SCOPUS:84929511557
SN - 0734-9750
VL - 33
SP - 358
EP - 369
JO - Biotechnology Advances
JF - Biotechnology Advances
IS - 3-4
ER -