Reduction of aflatoxins by Rhizopus oryzae and Trichoderma reesei

H. C.S. Hackbart*, A. R. Machado, A. Christ-Ribeiro, L. Prietto, E. Badiale-Furlong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

This study evaluated the ability of the microorganisms Rhizopus oryzae (CCT7560) and Trichoderma reesei (QM9414), producers of generally recognized as safe (GRAS) enzymes, to reduce the level of aflatoxins B1, B 2, G1, G2, and M1. The variables considered to the screening were the initial number of spores in the inoculum and the culture time. The culture was conducted in contaminated 4 % potato dextrose agar (PDA) medium, and the residual mycotoxins were determined every 24 h by HPLC-FL. The fungus R. oryzae has reduced aflatoxins B1, B 2, and G1 in the 96 h and aflatoxins M1 and G2 in the range of 120 h of culture by approximately 100 %. The fungus T. reesei has reduced aflatoxins B1, B2, and M 1 in the 96 h and aflatoxin G1 in the range of 120 h of culture by approximately 100 %. The highest reduction occurred in the middle of R. oryzae culture.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalMycotoxin Research
Volume30
Issue number3
DOIs
Publication statusPublished - Aug 2014

Keywords

  • Aflatoxins
  • Microorganisms
  • Mycotoxins

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