TY - JOUR
T1 - Reduction of antibiotic resistance determinants in urban wastewater by ozone
T2 - emphasis on the impact of wastewater matrix towards the inactivation kinetics, toxicity and bacterial regrowth
AU - Iakovides, I. C.
AU - Manoli, K.
AU - Karaolia, P.
AU - Michael-Kordatou, I.
AU - Manaia, C. M.
AU - Fatta-Kassinos, D.
N1 - Funding Information:
The present study was carried out in the context of the StARE project (KOINA/ΠΚΠ/0113/15), funded by the Cyprus Research Promotion Foundation (DESMI 2009–2010). Moreover, the study was a part of a project financed by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant agreement No 675530 . We thank the COST Action ES1403 NEREUS “New and emerging challenges and opportunities in wastewater reuse” supported by COST (European Cooperation in Science and Technology, www.cost.eu ) for providing the opportunity to collaborate. Iakovos C. Iakovides would like to warmly thank Vasiliki G. Beretsou (Nireas-IWRC) for her comments on the study, and Dr. Catarina Ferreira (Universidade Católica Portuguesa) for her valuable and constructive help during the analysis of genes and ARGs.
Funding Information:
The present study was carried out in the context of the StARE project (KOINA/???/0113/15), funded by the Cyprus Research Promotion Foundation (DESMI 2009?2010). Moreover, the study was a part of a project financed by the European Union's Horizon 2020 research and innovation programme under the Marie Sk?odowska-Curie Grant agreement No 675530. We thank the COST Action ES1403 NEREUS ?New and emerging challenges and opportunities in wastewater reuse? supported by COST (European Cooperation in Science and Technology, www.cost.eu) for providing the opportunity to collaborate. Iakovos C. Iakovides would like to warmly thank Vasiliki G. Beretsou (Nireas-IWRC) for her comments on the study, and Dr. Catarina Ferreira (Universidade Cat?lica Portuguesa) for her valuable and constructive help during the analysis of genes and ARGs.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/10/15
Y1 - 2021/10/15
N2 - This study investigated the impact of bench-scale ozonation on the inactivation of total cultivable and antibiotic-resistant bacteria (faecal coliforms, Escherichia coli, Pseudomonas aeruginosa, Enterococcus spp., and total heterotrophs), and the reduction of gene markers (16S rRNA and intl1) and antibiotic resistance genes (qacEΔ1, sul1, aadA1 and dfrA1) indigenously present in wastewater effluents treated by membrane bioreactor (MBR) or conventional activated sludge (CAS). The Chick-Watson model-predicted ozone exposure (CT) requirements, showed that higher CT values were needed for CAS- than MBR-treated effluents to achieve a 3-log reduction of each microbial group, i.e., ~30 and 10 gO3 min gDOC−1 respectively. Ozonation was efficient in inactivating the examined antibiotic-resistant bacteria, and no bacterial regrowth was observed after 72 h. The genes abundance decreased significantly by ozone, but an increase in their abundance was detected 72 h after storage of the treated samples. A very low removal of DOC was achieved and at the same time phyto- and eco-toxicity increased after the ozonation treatment in both wastewater matrices. The gene abundance, regrowth and toxicity results of this study may be of high environmental significance for comprehensive evaluation of ozone and may guide future studies in assessing these parameters for other oxidants/disinfectants.
AB - This study investigated the impact of bench-scale ozonation on the inactivation of total cultivable and antibiotic-resistant bacteria (faecal coliforms, Escherichia coli, Pseudomonas aeruginosa, Enterococcus spp., and total heterotrophs), and the reduction of gene markers (16S rRNA and intl1) and antibiotic resistance genes (qacEΔ1, sul1, aadA1 and dfrA1) indigenously present in wastewater effluents treated by membrane bioreactor (MBR) or conventional activated sludge (CAS). The Chick-Watson model-predicted ozone exposure (CT) requirements, showed that higher CT values were needed for CAS- than MBR-treated effluents to achieve a 3-log reduction of each microbial group, i.e., ~30 and 10 gO3 min gDOC−1 respectively. Ozonation was efficient in inactivating the examined antibiotic-resistant bacteria, and no bacterial regrowth was observed after 72 h. The genes abundance decreased significantly by ozone, but an increase in their abundance was detected 72 h after storage of the treated samples. A very low removal of DOC was achieved and at the same time phyto- and eco-toxicity increased after the ozonation treatment in both wastewater matrices. The gene abundance, regrowth and toxicity results of this study may be of high environmental significance for comprehensive evaluation of ozone and may guide future studies in assessing these parameters for other oxidants/disinfectants.
KW - Disinfection
KW - Ecotoxicity
KW - Genes
KW - Ozonation
KW - Phytotoxicity
UR - http://www.scopus.com/inward/record.url?scp=85111193456&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2021.126527
DO - 10.1016/j.jhazmat.2021.126527
M3 - Article
C2 - 34329111
AN - SCOPUS:85111193456
SN - 0304-3894
VL - 420
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 126527
ER -