TY - JOUR
T1 - Removal of microorganisms and antibiotic resistance genes from treated urban wastewater
T2 - a comparison between aluminium sulphate and tannin coagulants
AU - Grehs, Bárbara W.N.
AU - Lopes, Ana Rita
AU - Moreira, Nuno F.F.
AU - Fernandes, Telma
AU - Linton, Maria A.O.
AU - Silva, Adrián M.T.
AU - Manaia, Célia M.
AU - Carissimi, Elvis
AU - Nunes, Olga C.
N1 - Funding Information:
This work was financially supported by: project UID/EQU/00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE funded by national funds through FCT / MCTES (PIDDAC); project “LEPABE-2-ECO-INNOVATION” – NORTE-01-0145-FEDER-000005, funded by Norte Portugal Regional Operational Programme ( NORTE 2020 ), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) , and project Associate Laboratory LSRE-LCM-UID/EQU/50020/2019 - funded by national funds through FCT / MCTES (PIDDAC). Moreover, NFFM acknowledge financial support from FCT grant PD/BD/114318/2016 and we acknowledge support of Erasmus + IBRASIL Programme of the European Union . CMM and TF acknowledge the National Funds from FCT – Fundação para a Ciência e a Tecnologia through project WaterJPI/0001/2013 STARE – “Stopping Antibiotic Resistance Evolution” and UID/Multi/50016/2013.
Funding Information:
This work was financially supported by: project UID/EQU/00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy ? LEPABE funded by national funds through FCT/MCTES (PIDDAC); project ?LEPABE-2-ECO-INNOVATION? ? NORTE-01-0145-FEDER-000005, funded by Norte Portugal Regional Operational Programme (NORTE 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and project Associate Laboratory LSRE-LCM-UID/EQU/50020/2019 - funded by national funds through FCT/MCTES (PIDDAC). Moreover, NFFM acknowledge financial support from FCT grant PD/BD/114318/2016 and we acknowledge support of Erasmus + IBRASIL Programme of the European Union. CMM and TF acknowledge the National Funds from FCT ? Funda??o para a Ci?ncia e a Tecnologia through project WaterJPI/0001/2013 STARE ? ?Stopping Antibiotic Resistance Evolution? and UID/Multi/50016/2013.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The presence of antibiotic resistant-bacteria (ARB) and antibiotic resistance genes (ARG) in treated effluents of urban wastewater treatment plants (WWTP) may represent a threat to the environment and public health. Therefore, cost-effective technologies contributing to minimize loads of these contaminants in the final effluents of WWTP are required. This study aimed at assessing the capacity of coagulation to reduce the ARB&ARG load in secondary treated urban wastewater (STWW), as well as the impact of the process on the structure and diversity of the bacterial community. Coagulation performance using aluminium sulphate, a synthetic substance, and tannins, a biowaste, was compared. Samples were analysed immediately before (STWW) and after the coagulation treatment (Alu, Tan), as well as after 3-days storage in the dark at room temperature (RSTWW, RAlu, RTan), to assess possible reactivation events. Both coagulants decreased the turbidity and colour and reduced the bacterial load (16S rRNA gene copy number, total heterotrophs (HET), and ARB (faecal coliforms resistant to amoxicillin (FC/AMX) or ciprofloxacin (FC/CIP) up to 1–2 log immediately after the treatment. Both coagulants reduced the load of intl1, but in average, aluminium sulphate was able to decrease the content of the analysed ARGs (blaTEM and qnrS) to lower levels than tannin. Reactivation after storage was observed mainly in RTan. In these samples the load of the culturable populations and qnrS gene prevalence increased, sometimes to values higher than those found in the initial wastewater. Reactivation was also characterized by an increment in Gammaproteobacteria relative abundance in the bacterial community, although with distinct patterns for RTan and RAlu. Curvibacter, Undibacterium and Aquaspirillum were among the most abundant genera in RAlu and Aeromonas, Pseudomonas and Stenotrophomonas in RTan. These bacterial community shifts were in agreement with the variations in the culturable bacterial counts of HET for RTan and FC/CIP for RAlu. In summary, the overall performance of aluminium sulphate was better than that of tannins in the treatment of treated urban wastewater.
AB - The presence of antibiotic resistant-bacteria (ARB) and antibiotic resistance genes (ARG) in treated effluents of urban wastewater treatment plants (WWTP) may represent a threat to the environment and public health. Therefore, cost-effective technologies contributing to minimize loads of these contaminants in the final effluents of WWTP are required. This study aimed at assessing the capacity of coagulation to reduce the ARB&ARG load in secondary treated urban wastewater (STWW), as well as the impact of the process on the structure and diversity of the bacterial community. Coagulation performance using aluminium sulphate, a synthetic substance, and tannins, a biowaste, was compared. Samples were analysed immediately before (STWW) and after the coagulation treatment (Alu, Tan), as well as after 3-days storage in the dark at room temperature (RSTWW, RAlu, RTan), to assess possible reactivation events. Both coagulants decreased the turbidity and colour and reduced the bacterial load (16S rRNA gene copy number, total heterotrophs (HET), and ARB (faecal coliforms resistant to amoxicillin (FC/AMX) or ciprofloxacin (FC/CIP) up to 1–2 log immediately after the treatment. Both coagulants reduced the load of intl1, but in average, aluminium sulphate was able to decrease the content of the analysed ARGs (blaTEM and qnrS) to lower levels than tannin. Reactivation after storage was observed mainly in RTan. In these samples the load of the culturable populations and qnrS gene prevalence increased, sometimes to values higher than those found in the initial wastewater. Reactivation was also characterized by an increment in Gammaproteobacteria relative abundance in the bacterial community, although with distinct patterns for RTan and RAlu. Curvibacter, Undibacterium and Aquaspirillum were among the most abundant genera in RAlu and Aeromonas, Pseudomonas and Stenotrophomonas in RTan. These bacterial community shifts were in agreement with the variations in the culturable bacterial counts of HET for RTan and FC/CIP for RAlu. In summary, the overall performance of aluminium sulphate was better than that of tannins in the treatment of treated urban wastewater.
KW - ARB
KW - ARG
KW - Bacterial communities
KW - Bacterial regrowth
KW - Coagulation
UR - http://www.scopus.com/inward/record.url?scp=85071980541&partnerID=8YFLogxK
U2 - 10.1016/j.watres.2019.115056
DO - 10.1016/j.watres.2019.115056
M3 - Article
C2 - 31520811
AN - SCOPUS:85071980541
SN - 0043-1354
VL - 166
SP - 1
EP - 12
JO - Water Research
JF - Water Research
M1 - 115056
ER -
