Reprint of “Extracellular production of tellurium nanoparticles by the photosynthetic bacterium Rhodobacter capsulatus”

Roberto Borghese*, Marco Brucale, Gianuario Fortunato, Massimiliano Lanzi, Alessio Mezzi, Francesco Valle, Massimiliano Cavallini, Davide Zannoni

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The toxic oxyanion tellurite (TeO32−) is acquired by cells of Rhodobacter capsulatus grown anaerobically in the light, via acetate permease ActP2 and then reduced to Te0 in the cytoplasm as needle-like black precipitates. Interestingly, photosynthetic cultures of R. capsulatus can also generate Te0 nanoprecipitates (TeNPs) outside the cells upon addition of the redox mediator lawsone (2-hydroxy-1,4-naphtoquinone). TeNPs generation kinetics were monitored to define the optimal conditions to produce TeNPs as a function of various carbon sources and lawsone concentration. We report that growing cultures over a 10 days period with daily additions of 1 mM tellurite led to the accumulation in the growth medium of TeNPs with dimensions from 200 up to 600–700 nm in length as determined by atomic force microscopy (AFM). This result suggests that nucleation of TeNPs takes place over the entire cell growth period although the addition of new tellurium Te0 to pre-formed TeNPs is the main strategy used by R. capsulatus to generate TeNPs outside the cells. Finally, X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FT-IR) analysis of TeNPs indicate they are coated with an organic material which keeps the particles in solution in aqueous solvents.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalJournal of Hazardous Materials
Volume324
DOIs
Publication statusPublished - 15 Feb 2017
Externally publishedYes

Keywords

  • Lawsone
  • Nanoprecipitates
  • Photosynthetic bacteria
  • Rhodobacter capsulatus
  • Tellurite

Fingerprint

Dive into the research topics of 'Reprint of “Extracellular production of tellurium nanoparticles by the photosynthetic bacterium Rhodobacter capsulatus”'. Together they form a unique fingerprint.

Cite this