TY - JOUR
T1 - Response surface evaluation of microwave-assisted extraction conditions for Lycium barbarum bioactive compounds
AU - Mendes, Marta
AU - Carvalho, Ana P.
AU - Magalhães, Júlia M. C. S.
AU - Moreira, Manuela
AU - Guido, Luís
AU - Gomes, Ana M.
AU - Delerue-Matos, Cristina
N1 - Funding Information:
This work was financed by FEDER funds through CCDR-N, in the scope of project Operação NORTE-07-0124-FEDER-000069-Ciência do Alimento, and through FCT - Fundação para a Ciência e a Tecnologia, in the scope of project PEst-C/EQB/LA0006/2013. Authors Carvalho and Moreira acknowledge Project NORTE-07-0124-FEDER-000069. Author Moreira also acknowledges FCT for grant (SFRH/BPD/97049/2013).
Publisher Copyright:
© 2016 Elsevier Ltd. All rights reserved.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - The effects of microwave-assisted extraction (MAE) on the qualitative and quantitative composition of extracted bioactive phytochemicals are strongly dependent on specific operational conditions. The impact of temperature, extraction time, and solvent dielectric properties on the MAE of antioxidant compounds from goji berry is hereby reported and discussed. Results were evaluated in terms of antioxidant capacity (ascertained by ABTS and ORAC assays), complemented with quantification of total phenolics content (TPC), and characterization of extracts through HPLC. Furthermore, the effect of storage temperature was also studied. Results revealed that temperature and solvent properties exert a significant effect on TPC and ORAC assays, whereas irradiation time plays a key role in the preparation of extracts to be evaluated through ABTS. The effect of solvent properties is tentatively explained considering the dielectric properties of methanol and water, whereas the observed effect of temperature could be explained by its influence on the solubility of phenolic compounds. Industrial Relevance: A careful selection of operational conditions during MAE, designed for a specific antioxidant assay, allows an increase in the bioactivity of the resultant extracts, which may become important in terms of industrial applications.
AB - The effects of microwave-assisted extraction (MAE) on the qualitative and quantitative composition of extracted bioactive phytochemicals are strongly dependent on specific operational conditions. The impact of temperature, extraction time, and solvent dielectric properties on the MAE of antioxidant compounds from goji berry is hereby reported and discussed. Results were evaluated in terms of antioxidant capacity (ascertained by ABTS and ORAC assays), complemented with quantification of total phenolics content (TPC), and characterization of extracts through HPLC. Furthermore, the effect of storage temperature was also studied. Results revealed that temperature and solvent properties exert a significant effect on TPC and ORAC assays, whereas irradiation time plays a key role in the preparation of extracts to be evaluated through ABTS. The effect of solvent properties is tentatively explained considering the dielectric properties of methanol and water, whereas the observed effect of temperature could be explained by its influence on the solubility of phenolic compounds. Industrial Relevance: A careful selection of operational conditions during MAE, designed for a specific antioxidant assay, allows an increase in the bioactivity of the resultant extracts, which may become important in terms of industrial applications.
KW - Antioxidant capacity
KW - Goji berry
KW - MAE
KW - Response surface methodology
KW - Solvent dielectric properties
KW - Temperature
UR - http://www.scopus.com/inward/record.url?scp=84954271115&partnerID=8YFLogxK
U2 - 10.1016/j.ifset.2015.12.025
DO - 10.1016/j.ifset.2015.12.025
M3 - Article
AN - SCOPUS:84954271115
SN - 1466-8564
VL - 33
SP - 319
EP - 326
JO - Innovative Food Science and Emerging Technologies
JF - Innovative Food Science and Emerging Technologies
ER -