Sargassum muticum and osmundea pinnatifida enzymatic extracts: chemical, structural, and cytotoxic characterization

Dina Rodrigues, Ana R. Costa-Pinto, Sérgio Sousa, Marta W. Vasconcelos, Manuela M. Pintado, Leonel Pereira, Teresa A. P. Rocha-Santos, João P. da Costa, Artur M. S. Silva, Armando C. Duarte, Ana M. P. Gomes*, Ana C. Freitas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)
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Abstract

Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts. Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts. FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida. No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts. The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.
Original languageEnglish
Article number209
Number of pages15
JournalMarine Drugs
Volume17
Issue number4
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • Cytotoxicity
  • Enzymatic extracts
  • Ftir-atr
  • Minerals
  • Mono and polysaccharides
  • Nmr
  • Osmundea pinnatifida
  • Sargassum muticum

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