Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis

Fabianne Soares Lima; Luis Felipe Matos; Isnayra Kerolaynne Pacheco; Fernando Reis; João Victor Frazão Câmara; Josué Junior Araujo Pierote; José Milton Matos; Alessandra Ribeiro; Walter Moura; Ana Cristina Fialho

doi:10.1590/0104-1428.210018

Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis

Fabianne Soares Lima, Luis Felipe Matos, Isnayra Kerolaynne Pacheco, Fernando Reis, João Victor Frazão Câmara^*, Josué Junior Araujo Pierote, José Milton Matos, Alessandra Ribeiro, Walter Moura, Ana Cristina Fialho

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

10 Downloads

Abstract

To analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in contact with blood agar; Hemolytic activity test in sheep's blood - Polymer extract with red blood cells solution; Antimicrobial activity test - Solid polymer in direct contact with E. Coli and S. Aureus. For hemolytic tests, none of the samples showed hemolysis. Negative hemolytic activity is a good indicator, as the maintenance of the blood clot in the area of the lesion is essential for the formation of new tissue. For the antimicrobial activity test, no significant activity was observed against the bacteria used. The polymer is not toxic to red blood cells, being viable for clinical application as a matrix for tissue regeneration.

Original language	English
Article number	e2022003
Number of pages	5
Journal	Polimeros
Volume	32
Issue number	1
DOIs	https://doi.org/10.1590/0104-1428.210018
Publication status	Published - 31 Jan 2022

Keywords

Bone matrix
Materials testing
Tissue scaffolds

Access to Document

10.1590/0104-1428.210018Licence: CC BY

41513633Final published version, 408 KBLicence: CC BY

http://hdl.handle.net/10400.14/36877Licence: CC BY

Cite this

@article{d83171326af941fcb0837eac263ae67e,

title = "Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis",

abstract = "To analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in contact with blood agar; Hemolytic activity test in sheep's blood - Polymer extract with red blood cells solution; Antimicrobial activity test - Solid polymer in direct contact with E. Coli and S. Aureus. For hemolytic tests, none of the samples showed hemolysis. Negative hemolytic activity is a good indicator, as the maintenance of the blood clot in the area of the lesion is essential for the formation of new tissue. For the antimicrobial activity test, no significant activity was observed against the bacteria used. The polymer is not toxic to red blood cells, being viable for clinical application as a matrix for tissue regeneration.",

keywords = "Bone matrix, Materials testing, Tissue scaffolds",

author = "Lima, {Fabianne Soares} and Matos, {Luis Felipe} and Pacheco, {Isnayra Kerolaynne} and Fernando Reis and C{\^a}mara, {Jo{\~a}o Victor Fraz{\~a}o} and Pierote, {Josu{\'e} Junior Araujo} and Matos, {Jos{\'e} Milton} and Alessandra Ribeiro and Walter Moura and Fialho, {Ana Cristina}",

year = "2022",

month = jan,

day = "31",

doi = "10.1590/0104-1428.210018",

language = "English",

volume = "32",

journal = "Polimeros",

issn = "0104-1428",

publisher = "Associacao Brasileira de Polimeros",

number = "1",

}

TY - JOUR

T1 - Scaffold based on castor oil as an osteoconductive matrix in bone repair

T2 - biocompatibility analysis

AU - Lima, Fabianne Soares

AU - Matos, Luis Felipe

AU - Pacheco, Isnayra Kerolaynne

AU - Reis, Fernando

AU - Câmara, João Victor Frazão

AU - Pierote, Josué Junior Araujo

AU - Matos, José Milton

AU - Ribeiro, Alessandra

AU - Moura, Walter

AU - Fialho, Ana Cristina

PY - 2022/1/31

Y1 - 2022/1/31

N2 - To analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in contact with blood agar; Hemolytic activity test in sheep's blood - Polymer extract with red blood cells solution; Antimicrobial activity test - Solid polymer in direct contact with E. Coli and S. Aureus. For hemolytic tests, none of the samples showed hemolysis. Negative hemolytic activity is a good indicator, as the maintenance of the blood clot in the area of the lesion is essential for the formation of new tissue. For the antimicrobial activity test, no significant activity was observed against the bacteria used. The polymer is not toxic to red blood cells, being viable for clinical application as a matrix for tissue regeneration.

AB - To analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in contact with blood agar; Hemolytic activity test in sheep's blood - Polymer extract with red blood cells solution; Antimicrobial activity test - Solid polymer in direct contact with E. Coli and S. Aureus. For hemolytic tests, none of the samples showed hemolysis. Negative hemolytic activity is a good indicator, as the maintenance of the blood clot in the area of the lesion is essential for the formation of new tissue. For the antimicrobial activity test, no significant activity was observed against the bacteria used. The polymer is not toxic to red blood cells, being viable for clinical application as a matrix for tissue regeneration.

KW - Bone matrix

KW - Materials testing

KW - Tissue scaffolds

UR - http://www.scopus.com/inward/record.url?scp=85124316267&partnerID=8YFLogxK

U2 - 10.1590/0104-1428.210018

DO - 10.1590/0104-1428.210018

M3 - Article

AN - SCOPUS:85124316267

SN - 0104-1428

VL - 32

JO - Polimeros

JF - Polimeros

IS - 1

M1 - e2022003

ER -

Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this