Screening of bacteriocinogenic lactic acid bacteria cultures and their characterization as potential probiotics

Ana Pinto, Joana Barbosa, Helena Albano, Paula Teixeira*

*Corresponding author for this work

Research output: Contribution to conferencePoster

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Abstract

Introduction: Ingestion of probiotic lactic acid bacteria (LAB) has been pointed out to confer a range of health benefits, including the immune system stimulation, reduction of infectious illness, among others (Harzallah and Belhadj 2013). Food products containing probiotics should follow the guidelines established by FAO/WHO (2001). In general, probiotics have to be harmless to the host, they cannot be pathogenic, mutagenic or carcinogenic, allergenic or provoke any type of adverse reactions. For commercial purposes, potential probiotics need to be subjected to several tests: i) to the level of safety: origin, screening and selection of probiotics in terms of phenotype and genotype pathogenicity; ii) to the level of functionality: probiotics should be tolerant to acid and bile and possess intestinal epithelial adhesion properties; iii) to the level of technology: tests for genetically stable strains are required for large-scale production and iv) to the physiological level: immunomodulation, cholesterol metabolism, antimutagenic and anticarcinogenic properties are needed assays. The aim of this study was to select different bacteriocinogenic LAB isolated from different food products and study some of their probiotic characteristics in terms of safety, functional and physiological properties. Discussion & Conclusion: From all the 280 LAB isolates tested, only seven showed bacteriocinogenic activity and were selected for further studies. These isolates were identified as P. pentosaceus (6) and L. plantarum (1). All the isolates lacked intrinsic virulence factors and no antibiotic resistances were detected phenotypically. Also, all the isolates survived through the simulated gastrointestinal tract conditions, except L. plantarum R23, which growth was reduced in more than 2 log cycles. On the other hand, only P. pentosaceus CFF4 was able to adhere to Caco-2 cells (11 % of adherence after the simulated gastrointestinal tract passage). In conclusion, this study demonstrated that, at least one isolate, P. pentosaceus CFF4, presented the most attributes to be a potential probiotic bacterium; however, more studies must be carried out to validate this potential.
Original languageEnglish
Number of pages1
Publication statusPublished - 2017
EventMicrobiotec’17: Congress of Microbiology and Biotechnology - Universidade Católica Potuguesa, Porto, Portugal
Duration: 7 Dec 20179 Dec 2017
https://www.porto.ucp.pt/pt/microbiotec17#

Conference

ConferenceMicrobiotec’17
Country/TerritoryPortugal
CityPorto
Period7/12/179/12/17
Internet address

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