TY - JOUR
T1 - Separation and identification of neutral cereal lipids by normal phase high-performance liquid chromatography, using evaporative light-scattering and electrospray mass spectrometry for detection
AU - Rocha, João M.
AU - Kalo, Paavo J.
AU - Ollilainen, Velimatti
AU - Malcata, F. Xavier
PY - 2010/4
Y1 - 2010/4
N2 - A novel method was developed for the analysis of molecular species in neutral lipid classes, using separation by normal phase high-performance liquid chromatography, followed by detection by evaporative light-scattering and electrospray ionization tandem mass spectrometry. Monoacid standards, i.e. sterol esters, triacylglycerols, fatty acids, diacylglycerols, free sterols and monoacylglycerols, were separated to baseline on microbore 3μm-silica gel columns. Complete or partial separation of molecular species in each lipid class permitted identification by automatic tandem mass spectrometry of ammonium adducts, produced via positive electrospray ionization. After optimization of the method, separation and identification of molecular species of various lipid classes was comprehensively tested by analysis of neutral lipids from the free lipid extract of maize flour.
AB - A novel method was developed for the analysis of molecular species in neutral lipid classes, using separation by normal phase high-performance liquid chromatography, followed by detection by evaporative light-scattering and electrospray ionization tandem mass spectrometry. Monoacid standards, i.e. sterol esters, triacylglycerols, fatty acids, diacylglycerols, free sterols and monoacylglycerols, were separated to baseline on microbore 3μm-silica gel columns. Complete or partial separation of molecular species in each lipid class permitted identification by automatic tandem mass spectrometry of ammonium adducts, produced via positive electrospray ionization. After optimization of the method, separation and identification of molecular species of various lipid classes was comprehensively tested by analysis of neutral lipids from the free lipid extract of maize flour.
KW - Acylglycerols
KW - Evaporative light-scattering detection
KW - Fatty acids
KW - Free sterols
KW - Normal phase liquid chromatography
KW - Positive electrospray ionization tandem mass spectrometry
KW - Simple lipids
UR - http://www.scopus.com/inward/record.url?scp=77951207186&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2010.02.034
DO - 10.1016/j.chroma.2010.02.034
M3 - Article
C2 - 20346455
AN - SCOPUS:77951207186
SN - 0021-9673
VL - 1217
SP - 3013
EP - 3025
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 18
ER -