Sequential injection lab-on-valve system for the determination of the activity of peroxidase in vegetables

Susana S.M.P. Vidigal; Ildikó V. Tóth; António O.S.S. Rangel

doi:10.1021/jf9035113

Sequential injection lab-on-valve system for the determination of the activity of peroxidase in vegetables

Susana S.M.P. Vidigal, Ildikó V. Tóth, António O.S.S. Rangel

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Horseradish peroxidase (HRP) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. The objective of this work was to develop a methodology for the spectrophotometry determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential injection lab-on-valve format. The developed system is based on the reaction between hydrogen peroxide (H₂O₂) and 2,2-azinobis(3-ethylbenzothiazoline-6)sulfonic acid (ABTS) catalyzed by the enzyme (HRP). The method presented a sample consumption of 15νL. per assay and a consumption of ABTS and H₂O₂ of 24 μg and 12 μg per assay, respectively. It was also possible to monitor online the thermal inactivation of peroxidase at different temperature ranges.

Original language	English
Pages (from-to)	2071-2075
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	58
Issue number	4
DOIs	https://doi.org/10.1021/jf9035113
Publication status	Published - 24 Feb 2010

Keywords

Horseradish peroxidase
Sequential injection lab-on-valve
Spectrophotometry
Thermal inactlvatlon
Vegetables

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10.1021/jf9035113

http://hdl.handle.net/10400.14/2662

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title = "Sequential injection lab-on-valve system for the determination of the activity of peroxidase in vegetables",

abstract = "Horseradish peroxidase (HRP) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. The objective of this work was to develop a methodology for the spectrophotometry determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential injection lab-on-valve format. The developed system is based on the reaction between hydrogen peroxide (H2O2) and 2,2-azinobis(3-ethylbenzothiazoline-6)sulfonic acid (ABTS) catalyzed by the enzyme (HRP). The method presented a sample consumption of 15νL. per assay and a consumption of ABTS and H2O2 of 24 μg and 12 μg per assay, respectively. It was also possible to monitor online the thermal inactivation of peroxidase at different temperature ranges.",

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T1 - Sequential injection lab-on-valve system for the determination of the activity of peroxidase in vegetables

AU - Vidigal, Susana S.M.P.

AU - Tóth, Ildikó V.

AU - Rangel, António O.S.S.

PY - 2010/2/24

Y1 - 2010/2/24

N2 - Horseradish peroxidase (HRP) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. The objective of this work was to develop a methodology for the spectrophotometry determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential injection lab-on-valve format. The developed system is based on the reaction between hydrogen peroxide (H2O2) and 2,2-azinobis(3-ethylbenzothiazoline-6)sulfonic acid (ABTS) catalyzed by the enzyme (HRP). The method presented a sample consumption of 15νL. per assay and a consumption of ABTS and H2O2 of 24 μg and 12 μg per assay, respectively. It was also possible to monitor online the thermal inactivation of peroxidase at different temperature ranges.

AB - Horseradish peroxidase (HRP) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. The objective of this work was to develop a methodology for the spectrophotometry determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential injection lab-on-valve format. The developed system is based on the reaction between hydrogen peroxide (H2O2) and 2,2-azinobis(3-ethylbenzothiazoline-6)sulfonic acid (ABTS) catalyzed by the enzyme (HRP). The method presented a sample consumption of 15νL. per assay and a consumption of ABTS and H2O2 of 24 μg and 12 μg per assay, respectively. It was also possible to monitor online the thermal inactivation of peroxidase at different temperature ranges.

KW - Horseradish peroxidase

KW - Sequential injection lab-on-valve

KW - Spectrophotometry

KW - Thermal inactlvatlon

KW - Vegetables

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JF - Journal of Agricultural and Food Chemistry

IS - 4

ER -

Sequential injection lab-on-valve system for the determination of the activity of peroxidase in vegetables

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Keywords

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