Sequential injection system for the enzymatic determination of ethanol in alcoholic beverages with in-line dilution

Teresa F. M. Pais, Susana S. M. P. Vidigal, Ildikó V. Tóth, António O. S. S. Rangel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A sequential injection system for the enzymatic determination of ethanol in wines with spectrophotometric detection was developed. The determination was based on the enzymatic reaction involving ethanol and NAD+ in the presence of alcohol dehydrogenase. A gas-diffusion unit was introduced in the system to perform an in-line dilution. It was possible to operate the system with a reagent consumption of 1.3 mg of NAD+, 0.45 U of ADH, and 32 μL of sample, per assay with a determination rate of 21 h-1.The results obtained from the analysis of wine samples were statistically equivalent to those obtained by the reference method, with good repeatability; the relative standard deviation (n = 10) was <1% with a LOQ of 0.3% (v/v).
Original languageEnglish
Pages (from-to)616-620
Number of pages5
JournalFood Control
Volume30
Issue number2
DOIs
Publication statusPublished - 1 Apr 2013

Keywords

  • Alcohol dehydrogenase
  • Ethanol determination
  • Gas-diffusion unit
  • Sequential injection analysis system
  • Spectrophotometry
  • Wine

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