Sequential injection system for the enzymatic determination of ethanol in wine

Ricardo N. M. J. Páscoa; Susana S. M. P. Vidigal; Ildikó V. Tóth; António O. S. S. Rangel

doi:10.1021/jf051787l

Sequential injection system for the enzymatic determination of ethanol in wine

Ricardo N. M. J. Páscoa, Susana S. M. P. Vidigal, Ildikó V. Tóth, António O. S. S. Rangel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD⁺. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn=10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h ^-1; 1 μmol of NAD⁺, 1.1 units of enzyme, and 50 μL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.

Original language	English
Pages (from-to)	19-23
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	54
Issue number	1
DOIs	https://doi.org/10.1021/jf051787l
Publication status	Published - 11 Jan 2006

Keywords

Alcohol dehydrogenase
Ethanol
Sequential injection analysis
Spectrophotometry
Wine

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10.1021/jf051787l

http://hdl.handle.net/10400.14/6786

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title = "Sequential injection system for the enzymatic determination of ethanol in wine",

abstract = "A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn=10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h -1; 1 μmol of NAD+, 1.1 units of enzyme, and 50 μL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.",

keywords = "Alcohol dehydrogenase, Ethanol, Sequential injection analysis, Spectrophotometry, Wine",

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doi = "10.1021/jf051787l",

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T1 - Sequential injection system for the enzymatic determination of ethanol in wine

AU - Páscoa, Ricardo N. M. J.

AU - Vidigal, Susana S. M. P.

AU - Tóth, Ildikó V.

AU - Rangel, António O. S. S.

PY - 2006/1/11

Y1 - 2006/1/11

N2 - A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn=10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h -1; 1 μmol of NAD+, 1.1 units of enzyme, and 50 μL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.

AB - A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn=10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h -1; 1 μmol of NAD+, 1.1 units of enzyme, and 50 μL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.

KW - Alcohol dehydrogenase

KW - Ethanol

KW - Sequential injection analysis

KW - Spectrophotometry

KW - Wine

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EP - 23

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

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ER -

Sequential injection system for the enzymatic determination of ethanol in wine

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Keywords

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