Serum Response Factor Is Required for Sprouting Angiogenesis and Vascular Integrity

Claudio Areias Franco; Mathias Mericskay; Ara Parlakian; Guillaume Gary-Bobo; Jacqueline Gao-Li; Denise Paulin; Erika Gustafsson; Zhenlin Li

doi:10.1016/j.devcel.2008.07.019

Serum Response Factor Is Required for Sprouting Angiogenesis and Vascular Integrity

Claudio Areias Franco, Mathias Mericskay, Ara Parlakian, Guillaume Gary-Bobo, Jacqueline Gao-Li, Denise Paulin, Erika Gustafsson, Zhenlin Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Serum response factor (SRF) is a transcription factor that controls the expression of cytoskeletal proteins and immediate early genes in different cell types. Here, we found that SRF expression is restricted to endothelial cells (ECs) of small vessels such as capillaries in the mouse embryo. EC-specific Srf deletion led to aneurysms and hemorrhages from 11.5 days of mouse development (E11.5) and lethality at E14.5. Mutant embryos presented a reduced capillary density and defects in EC migration, with fewer numbers of filopodia in tip cells and ECs showing defects in actin polymerization and intercellular junctions. We show that SRF is essential for the expression of VE-cadherin and β-actin in ECs both in vivo and in vitro. Moreover, knockdown of SRF in ECs impaired VEGF- and FGF-induced in vitro angiogenesis. Taken together, our results demonstrate that SRF plays an important role in sprouting angiogenesis and small vessel integrity in the mouse embryo.

Original language	English
Pages (from-to)	448-461
Number of pages	14
Journal	Developmental Cell
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/j.devcel.2008.07.019
Publication status	Published - 16 Sept 2008
Externally published	Yes

Keywords

CELLBIO
DEVBIO

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.devcel.2008.07.019

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title = "Serum Response Factor Is Required for Sprouting Angiogenesis and Vascular Integrity",

abstract = "Serum response factor (SRF) is a transcription factor that controls the expression of cytoskeletal proteins and immediate early genes in different cell types. Here, we found that SRF expression is restricted to endothelial cells (ECs) of small vessels such as capillaries in the mouse embryo. EC-specific Srf deletion led to aneurysms and hemorrhages from 11.5 days of mouse development (E11.5) and lethality at E14.5. Mutant embryos presented a reduced capillary density and defects in EC migration, with fewer numbers of filopodia in tip cells and ECs showing defects in actin polymerization and intercellular junctions. We show that SRF is essential for the expression of VE-cadherin and β-actin in ECs both in vivo and in vitro. Moreover, knockdown of SRF in ECs impaired VEGF- and FGF-induced in vitro angiogenesis. Taken together, our results demonstrate that SRF plays an important role in sprouting angiogenesis and small vessel integrity in the mouse embryo.",

keywords = "CELLBIO, DEVBIO",

author = "Franco, {Claudio Areias} and Mathias Mericskay and Ara Parlakian and Guillaume Gary-Bobo and Jacqueline Gao-Li and Denise Paulin and Erika Gustafsson and Zhenlin Li",

note = "Funding Information: C.A.F. was supported by the Fondation pour la Recherche Medicale. We thank A. Eichmann, D. Daegelen, D. Tuil, A. Janin, A. Grosfeld, and S. Germain for fruitful discussion and suggestions; B. el-Zhalek for assistance with electronic microscope analysis; and C. Freitas and E. Gomez for their generous gift of antibodies. This work was supported by l'Agence Nationale pour la Recherche (COD2005), La Fondation de France, and Association Fran{\c c}aise contre les Myopathies. ",

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doi = "10.1016/j.devcel.2008.07.019",

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AU - Franco, Claudio Areias

AU - Mericskay, Mathias

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AU - Gao-Li, Jacqueline

AU - Paulin, Denise

AU - Gustafsson, Erika

AU - Li, Zhenlin

N1 - Funding Information: C.A.F. was supported by the Fondation pour la Recherche Medicale. We thank A. Eichmann, D. Daegelen, D. Tuil, A. Janin, A. Grosfeld, and S. Germain for fruitful discussion and suggestions; B. el-Zhalek for assistance with electronic microscope analysis; and C. Freitas and E. Gomez for their generous gift of antibodies. This work was supported by l'Agence Nationale pour la Recherche (COD2005), La Fondation de France, and Association Française contre les Myopathies.

PY - 2008/9/16

Y1 - 2008/9/16

N2 - Serum response factor (SRF) is a transcription factor that controls the expression of cytoskeletal proteins and immediate early genes in different cell types. Here, we found that SRF expression is restricted to endothelial cells (ECs) of small vessels such as capillaries in the mouse embryo. EC-specific Srf deletion led to aneurysms and hemorrhages from 11.5 days of mouse development (E11.5) and lethality at E14.5. Mutant embryos presented a reduced capillary density and defects in EC migration, with fewer numbers of filopodia in tip cells and ECs showing defects in actin polymerization and intercellular junctions. We show that SRF is essential for the expression of VE-cadherin and β-actin in ECs both in vivo and in vitro. Moreover, knockdown of SRF in ECs impaired VEGF- and FGF-induced in vitro angiogenesis. Taken together, our results demonstrate that SRF plays an important role in sprouting angiogenesis and small vessel integrity in the mouse embryo.

AB - Serum response factor (SRF) is a transcription factor that controls the expression of cytoskeletal proteins and immediate early genes in different cell types. Here, we found that SRF expression is restricted to endothelial cells (ECs) of small vessels such as capillaries in the mouse embryo. EC-specific Srf deletion led to aneurysms and hemorrhages from 11.5 days of mouse development (E11.5) and lethality at E14.5. Mutant embryos presented a reduced capillary density and defects in EC migration, with fewer numbers of filopodia in tip cells and ECs showing defects in actin polymerization and intercellular junctions. We show that SRF is essential for the expression of VE-cadherin and β-actin in ECs both in vivo and in vitro. Moreover, knockdown of SRF in ECs impaired VEGF- and FGF-induced in vitro angiogenesis. Taken together, our results demonstrate that SRF plays an important role in sprouting angiogenesis and small vessel integrity in the mouse embryo.

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Serum Response Factor Is Required for Sprouting Angiogenesis and Vascular Integrity

Abstract

Keywords

UN SDGs

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