Shinella fusca sp. nov., isolated from domestic waste compost

A bacterium, designated strain DC-196^T, isolated from kitchen refuse compost was analysed by using a polyphasic approach. Strain DC-196^T was characterized as a Gram-negative short rod that was catalase- and oxidase-positive, and able to grow at 10-40 °C, pH 6-9 and in NaCl concentrations as high as 3%. Chemotaxonomically, C_18:1 was observed to be the predominant cellular fatty acid and ubiquinone 10 (Q10) was the predominant respiratory quinone. The G+C content of the genomic DNA was determined to be 66 mol%. On the basis of the genotypic, phenotypic and chemotaxonomic characteristics, strain DC-196^T was assigned to the genus Shinella, although with distinctive features. At the time of writing, 16S rRNA gene sequence similarities of 97.6-96.8% and the low DNA-DNA hybridization values of 38.2-32.2% with the type strains of the three recognized Shinella species confirmed that strain DC-196^T represents a novel species of the genus, for which the name Shinella fusca sp. nov. is proposed (type strain DC-196^T=CCUG 55808^T=LMG 24714^T).