TY - JOUR
T1 - Short-communication
T2 - study of fatty acid metabolites in microbial conjugated fatty acids-enrichment of milk and discovery of additional undescribed conjugated linolenic acid isomers
AU - Fontes, Ana Luiza
AU - Neves, Bruna
AU - Conde, Tiago
AU - Couto, Daniela
AU - Pimentel, Lígia Leão
AU - Rodríguez-Alcalá, Luis Miguel
AU - Domingues, M. Rosário
AU - Gomes, Ana Maria
N1 - Funding Information:
This work was financed by National funds via FCT – Fundação para a Ciência e a Tecnologia, under the project “Pro-TECh-CLnA - Microbial Production of Bioactive Conjugated Linolenic Acid Isomers to Obtain Functional Ingredients and Foods” reference PTDC/AGR-TEC/2125/2014. We would also like to thank the scientific collaboration under the FCT project UIDB/50016/2020. Financial support for the author A.L. Fontes was provided by the scholarship SFRH/BD/117721/2016 granted by the Portuguese government through FCT. Tiago Conde (2020.05678.BD), Bruna Neves (2021.04602.BD) and Daniela Couto (SFRH/BD/138992/2018) are thankful to FCT for their PhD grant. Thanks are due to the University of Aveiro and FCT/MCT and FCT/MEC (PIDDAC) for the financial support to CESAM (UIDB/50017/2020 + UIDP/50017/2020), LAQV/REQUIMTE (UIDB/50006/2020), and to RNEM, Portuguese Mass Spectrometry Network, (LISBOA-01-0145-FEDER-402-022125) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement.
Funding Information:
This work was financed by National funds via FCT – Fundação para a Ciência e a Tecnologia, under the project “Pro-TECh-CLnA - Microbial Production of Bioactive Conjugated Linolenic Acid Isomers to Obtain Functional Ingredients and Foods” reference PTDC/AGR-TEC/2125/2014. We would also like to thank the scientific collaboration under the FCT project UIDB/50016/2020. Financial support for the author A.L. Fontes was provided by the scholarship SFRH/BD/117721/2016 granted by the Portuguese government through FCT. Tiago Conde (2020.05678.BD), Bruna Neves (2021.04602.BD) and Daniela Couto (SFRH/BD/138992/2018) are thankful to FCT for their PhD grant. Thanks are due to the University of Aveiro and FCT/MCT and FCT/MEC (PIDDAC) for the financial support to CESAM (UIDB/50017/2020 + UIDP/50017/2020), LAQV/REQUIMTE (UIDB/50006/2020), and to RNEM, Portuguese Mass Spectrometry Network, (LISBOA-01-0145-FEDER-402-022125) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement.
Publisher Copyright:
© 2023 The Author(s)
PY - 2024/1/30
Y1 - 2024/1/30
N2 - Microbially enriched food in conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids is intensively studied nowadays. The conversion of linoleic (LA) and α-linolenic acids (α-LNA) into these compounds may involve different fatty acid (FA) intermediates. This research aimed to investigate potential FA byproducts in milk during microbial CLA/CLNA-enrichment using Bifidobacterium breve DSM 20091. Milk fermented with pure α-LNA showed a decrease in free myristic acid, while pure LA led to an increase in free stearic acid. No additional FA compounds were found alongside CLA/CLNA isomers. The strain produced several CLA isomers from LA, but only when administered alone. Nonetheless, when α-LNA was assayed, additional CLNA isomers, never reported before for bifidobacteria, were observed. In conclusion, except for stearic acid in the presence of LA, no side-FA metabolites were released during milk microbial CLA/CLNA-enrichment. Results suggest either CLA/CLNA production occurs in one single-step or intermediates biotransformation is very fast.
AB - Microbially enriched food in conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids is intensively studied nowadays. The conversion of linoleic (LA) and α-linolenic acids (α-LNA) into these compounds may involve different fatty acid (FA) intermediates. This research aimed to investigate potential FA byproducts in milk during microbial CLA/CLNA-enrichment using Bifidobacterium breve DSM 20091. Milk fermented with pure α-LNA showed a decrease in free myristic acid, while pure LA led to an increase in free stearic acid. No additional FA compounds were found alongside CLA/CLNA isomers. The strain produced several CLA isomers from LA, but only when administered alone. Nonetheless, when α-LNA was assayed, additional CLNA isomers, never reported before for bifidobacteria, were observed. In conclusion, except for stearic acid in the presence of LA, no side-FA metabolites were released during milk microbial CLA/CLNA-enrichment. Results suggest either CLA/CLNA production occurs in one single-step or intermediates biotransformation is very fast.
KW - Bifidobacterium breve
KW - Conjugated linoleic acid
KW - Conjugated linolenic acid
KW - Fatty acid metabolites
UR - http://www.scopus.com/inward/record.url?scp=85169783653&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2023.137276
DO - 10.1016/j.foodchem.2023.137276
M3 - Article
C2 - 37659326
SN - 0308-8146
VL - 432
JO - Food Chemistry
JF - Food Chemistry
M1 - 137276
ER -