Short-communication: study of fatty acid metabolites in microbial conjugated fatty acids-enrichment of milk and discovery of additional undescribed conjugated linolenic acid isomers

Ana Luiza Fontes, Bruna Neves, Tiago Conde, Daniela Couto, Lígia Leão Pimentel, Luis Miguel Rodríguez-Alcalá*, M. Rosário Domingues, Ana Maria Gomes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Microbially enriched food in conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids is intensively studied nowadays. The conversion of linoleic (LA) and α-linolenic acids (α-LNA) into these compounds may involve different fatty acid (FA) intermediates. This research aimed to investigate potential FA byproducts in milk during microbial CLA/CLNA-enrichment using Bifidobacterium breve DSM 20091. Milk fermented with pure α-LNA showed a decrease in free myristic acid, while pure LA led to an increase in free stearic acid. No additional FA compounds were found alongside CLA/CLNA isomers. The strain produced several CLA isomers from LA, but only when administered alone. Nonetheless, when α-LNA was assayed, additional CLNA isomers, never reported before for bifidobacteria, were observed. In conclusion, except for stearic acid in the presence of LA, no side-FA metabolites were released during milk microbial CLA/CLNA-enrichment. Results suggest either CLA/CLNA production occurs in one single-step or intermediates biotransformation is very fast.
Original languageEnglish
Article number137276
Number of pages17
JournalFood Chemistry
Volume432
DOIs
Publication statusPublished - 30 Jan 2024

Keywords

  • Bifidobacterium breve
  • Conjugated linoleic acid
  • Conjugated linolenic acid
  • Fatty acid metabolites

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