Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion

Ana Margarida Pereira, Raul Machado*, André da Costa, Artur Ribeiro, Tony Collins, Andreia C. Gomes, Isabel B. Leonor, David L. Kaplan, Rui L. Reis, Margarida Casal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

The objective of this work was to exploit the fibronectin type II (FNII) module from human matrix metalloproteinase-2 as a functional domain for the development of silk-based biopolymer blends that display enhanced cell adhesion properties. The DNA sequence of spider dragline silk protein (6mer) was genetically fused with the FNII coding sequence and expressed in Escherichia coli. The chimeric protein 6mer + FNII was purified by non-chromatographic methods. Films prepared from 6mer + FNII by solvent casting promoted only limited cell adhesion of human skin fibroblasts. However, the performance of the material in terms of cell adhesion was significantly improved when 6mer + FNII was combined with a silk-elastin-like protein in a concentration-dependent behavior. With this work we describe a novel class of biopolymer that promote cell adhesion and potentially useful as biomaterials for tissue engineering and regenerative medicine.
Original languageEnglish
Pages (from-to)50-59
Number of pages10
JournalActa Biomaterialia
Volume47
DOIs
Publication statusPublished - 1 Jan 2017
Externally publishedYes

Keywords

  • Fibronectin type II
  • Cell adhesion
  • Silk
  • Elastin
  • Biomaterials

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