TY - JOUR
T1 - Silk-based microcarriers
T2 - current developments and future perspectives
AU - Veiga, Anabela
AU - Castro, Filipa
AU - Rocha, Fernando
AU - Oliveira, Ana
N1 - Funding Information:
This work was financially supported by Base Funding – UIDB/ 00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE – funded by national funds through the FCT/MCTES (PIDDAC). The authors also acknowledge Portuguese National Funds from FCT – Fundação para a Ciência e a Tecnologia through project UID/ Multi/04044/2019. This work was supported by project Biotherapies-Bioengineered Therapies for Infectious Diseases and Tissue Regeneration and Interreg V-A POCTEP Programme through FEDER funds from the European Union [0245_IBEROS_1_E] C. A.
Funding Information:
This work was financially supported by Base Funding ? UIDB/ 00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy ? LEPABE ? funded by national funds through the FCT/MCTES (PIDDAC). The authors also acknowledge Portuguese National Funds from FCT ? Funda??o para a Ci?ncia e a Tecnologia through project UID/ Multi/04044/2019. This work was supported by project Biotherapies-Bioengineered Therapies for Infectious Diseases and Tissue Regeneration and Interreg V-A POCTEP Programme through FEDER funds from the European Union [0245_IBEROS_1_E] C. A.
Publisher Copyright:
© The Institution of Engineering and Technology 2020
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Cell-seeded microcarriers (MCs) are currently one of the most promising topics in biotechnology. These systems are supportive structures for cell growth and expansion that allow efficient nutrient and gas transfer between the media and the attached cells. Silk proteins have been increasingly used for this purpose in the past few years due to their biocompatibility, biodegradability and non-toxicity. To date, several silk fibroin spherical MCs in combination with alginate, gelatin and calcium phosphates have been reported with very interesting outcomes. In addition, other silk-based three-dimensional structures such as microparticles with chitosan and collagen, as well as organoids, have been increasingly studied. In this study, the physicochemical and biological properties of these biomaterials, as well as the recent methodologies for their processing and for cell culture, are discussed. The potential biomedical applications are also addressed. In addition, an analysis of the future perspectives is presented, where the potential of innovative silk-based MCs processing technologies is highlighted.
AB - Cell-seeded microcarriers (MCs) are currently one of the most promising topics in biotechnology. These systems are supportive structures for cell growth and expansion that allow efficient nutrient and gas transfer between the media and the attached cells. Silk proteins have been increasingly used for this purpose in the past few years due to their biocompatibility, biodegradability and non-toxicity. To date, several silk fibroin spherical MCs in combination with alginate, gelatin and calcium phosphates have been reported with very interesting outcomes. In addition, other silk-based three-dimensional structures such as microparticles with chitosan and collagen, as well as organoids, have been increasingly studied. In this study, the physicochemical and biological properties of these biomaterials, as well as the recent methodologies for their processing and for cell culture, are discussed. The potential biomedical applications are also addressed. In addition, an analysis of the future perspectives is presented, where the potential of innovative silk-based MCs processing technologies is highlighted.
UR - http://www.scopus.com/inward/record.url?scp=85094866794&partnerID=8YFLogxK
U2 - 10.1049/iet-nbt.2020.0058
DO - 10.1049/iet-nbt.2020.0058
M3 - Conference article
C2 - 33108319
AN - SCOPUS:85094866794
SN - 1751-8741
VL - 14
SP - 645
EP - 653
JO - IET Nanobiotechnology
JF - IET Nanobiotechnology
IS - 8
ER -