Silk fibroin/nano-CaP bilayered scaffolds for osteochondral tissue engineering

Le Ping Yan, Joaquim M. Oliveira, Ana L. Oliveira, Rui L. Reis*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

20 Citations (Scopus)

Abstract

In this study, bilayered silk and silk/nano-CaP scaffolds were developed for osteochondral (OC) tissue engineering. Aqueous silk solution (16 wt.%) was used for preparation of the cartilage-like layer and, for generation of the silk/nano-CaP suspension and the bottom layer (CaP/Silk: 16 wt.%). The scaffolds were formed by using salt-leaching/lyophilization approach. The scanning electron microscopy revealed that the both layers presented porous structure and integrated well. Micro-computed tomography images confirmed that the CaP phase was only retained in the silk/nano-CaP layer. The hydration degree and mechanical properties of the bilayered scaffold were comparable to the ones of each single layer. The apatite crystal formation was limited to the silk/nano-CaP layer, when soaking the scaffold in a simulated body fluid solution, which is a must for the application of the developed scaffolds in OC tissue engineering.
Original languageEnglish
Title of host publicationBioceramics 25
PublisherTrans Tech Publications Ltd
Pages245-248
Number of pages4
ISBN (Print)9783037858998
DOIs
Publication statusPublished - 2014
Event25th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2013 - Bucharest, Romania
Duration: 7 Nov 201310 Nov 2013

Publication series

NameKey Engineering Materials
Volume587
ISSN (Print)1013-9826
ISSN (Electronic)1662-9795

Conference

Conference25th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2013
Country/TerritoryRomania
CityBucharest
Period7/11/1310/11/13

Keywords

  • Bilayered scaffold
  • Nano calcium phosphate
  • Osteochondral tissue engineering
  • Silk fibroin

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