TY - JOUR
T1 - Silver-containing calcium phosphate materials of marine origin with antibacterial activity
AU - Piccirillo, C.
AU - Pullar, R. C.
AU - Tobaldi, D. M.
AU - Castro, P. M. Lima
AU - Pintado, M. M. Estevez
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Hydroxyapatite (Ca10(PO4)6(OH)2 - HAp) is a common material for bone replacement in artificial implants and prostheses, due to its high biocompatibility. In this work we report about the preparation of HAp-based materials of marine origin with antibacterial properties. Bones from cod fish (Gadhus morua) were treated with a dilute AgNO3 solution, to obtain HAp-containing samples with silver as a minor component. The samples were characterised by XRD, FT-IR spectroscopy and SEM, indicating the presence of HAp, β-TCP and some metallic silver. The determination of unit cell parameters, however, indicated that the majority of silver is substituted in the β-TCP lattice in ionic form. The antibacterial activity of these materials was tested towards both Gram-positive and Gram-negative bacterias; results showed that the material is very effective with Gram-negative strains such as Escherichia coli (inactivation rates of 99.82% and 99.999% after 2 and 5 h, respectively) and had a smaller but still significant effect on Gram-positive MRSA (91% inactivation rate). These results show the potentials of these samples as infection-resistant bone replacement materials.
AB - Hydroxyapatite (Ca10(PO4)6(OH)2 - HAp) is a common material for bone replacement in artificial implants and prostheses, due to its high biocompatibility. In this work we report about the preparation of HAp-based materials of marine origin with antibacterial properties. Bones from cod fish (Gadhus morua) were treated with a dilute AgNO3 solution, to obtain HAp-containing samples with silver as a minor component. The samples were characterised by XRD, FT-IR spectroscopy and SEM, indicating the presence of HAp, β-TCP and some metallic silver. The determination of unit cell parameters, however, indicated that the majority of silver is substituted in the β-TCP lattice in ionic form. The antibacterial activity of these materials was tested towards both Gram-positive and Gram-negative bacterias; results showed that the material is very effective with Gram-negative strains such as Escherichia coli (inactivation rates of 99.82% and 99.999% after 2 and 5 h, respectively) and had a smaller but still significant effect on Gram-positive MRSA (91% inactivation rate). These results show the potentials of these samples as infection-resistant bone replacement materials.
KW - Antibacterial
KW - Natural hydroxyapatite
KW - Silver
KW - β-tricalcium phosphate
UR - http://www.scopus.com/inward/record.url?scp=84930925393&partnerID=8YFLogxK
U2 - 10.1016/j.ceramint.2015.04.116
DO - 10.1016/j.ceramint.2015.04.116
M3 - Article
AN - SCOPUS:84930925393
SN - 0272-8842
VL - 41
SP - 10152
EP - 10159
JO - Ceramics International
JF - Ceramics International
IS - 8
M1 - 10523
ER -