@article{9eb480b90abf4160aec2bf154b6f28a4,
title = "Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer",
abstract = "Genetically engineered protein polymers functionalized with bioactive domains have potential as multifunctional versatile materials for biomedical use. The present work describes the fabrication and characterisation of antimicrobial fibre mats comprising the antimicrobial elastin-like recombinamer (ELR) CM4-A200. The CM4-A200 protein polymer derives from the genetic fusion of the ABP-CM4 antimicrobial peptide from Bombyx mori with 200 repetitions of the pentamer VPAVG. This is the first report on non-crosslinked fibre mats fabricated with an antimicrobial ELR stable in solution. Thermal gravimetric analysis of CM4-A200 fibre mats shows one single degradation step at temperatures above 300 °C, with fibres displaying a higher thermal degradation activation. The electrospun CM4-A200 fibres display high antimicrobial activity against Gram-positive and Gram-negative bacteria with no detectable cytotoxic effects against normal human skin fibroblasts and keratinocytes, revealing the great potential of these polymers for the fabrication of biomedical materials.",
author = "{Da Costa}, A. and Pereira, {A. M.} and Gomes, {A. C.} and Rodriguez-Cabello, {J. C.} and V. Sencadas and M. Casal and R. MacHado",
note = "Funding Information: This work was financed by the strategic programme UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT) and by the ERDF through the COMPETE2020—Programa Operacional Competiti-vidade e Internacionaliza{\c c}{\~a}o (POCI). The present work was also supported by FCT within the ERA-NET IB, project FunBioPlas with grant number ERA-IB-15-089 and FCT reference ERA-IB-2-6/0004/2014. AC and RM acknowledge FCT for SFRH/BD/75882/2011 and SFRH-BPD/86470/2012 grants, respectively. This article is a result of the project EcoAgriFood (NORTE-01-0145-FEDER-000009), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). The authors also acknowledge the Spanish Minister of Economy and Competitiveness (MAT2013-41723-R, MAT2013-42473-R and MAT2012-38043) and Junta de Castilla y Le{\'o}n-JCyL (VA244U13, VA313U14), Spain. Publisher Copyright: {\textcopyright} 2017 IOP Publishing Ltd.",
year = "2017",
month = jul,
day = "12",
doi = "10.1088/1748-605X/aa7104",
language = "English",
volume = "12",
journal = "Biomedical Materials (Bristol)",
issn = "1748-6041",
publisher = "IOP Publishing Ltd.",
number = "4",
}