Skin substitutes, methods and uses thereof

The present disclosure relates to a method to obtain a decellularized dermal matrix from a rabbit, hare or beaver skin graft, the method comprising the following steps: degreasing the skin graft using a hydrocarbon solvent, perchloroethylene, modified alcohols or supercritical carbon dioxide, or combinations thereof; liming the degreased graft by immersing the graft in a solution comprising calcium hydroxide, sodium hydroxide, hydrogen peroxide, or mixtures thereof; fleshing the skin graft; optionally, deliming the skin graft; incubating the skin graft in a solution of ethylenediamine tetraacetic acid; treating the skin graft with a detergent solution for 30 025/004023 A minutes to 3 hours to obtain the decellularized dermal matrix. The disclosure also relates to a decellularized dermal matrix obtained by the method described; to a composition comprising said decellularized cell matrix for use in medicine or veterinary medicine; and to dermo-epidermal skin substitute scaffold comprising the disclosed decellularized dermal matrix.