TY - JOUR
T1 - Solar treatment (H2O2, TiO2-P25 and GO-TiO2 photocatalysis, photo-Fenton) of organic micropollutants, human pathogen indicators, antibiotic resistant bacteria and related genes in urban wastewater
AU - Moreira, Nuno F. F.
AU - Narciso-da-Rocha, Carlos
AU - Polo-López, M. Inmaculada
AU - Pastrana-Martínez, Luisa M.
AU - Faria, Joaquim L.
AU - Manaia, Célia M.
AU - Fernández-Ibáñez, Pilar
AU - Nunes, Olga C.
AU - Silva, Adrián M. T.
N1 - Funding Information:
This work was financially supported by Project nº P1404290052 under the SFERA Program (EC/FP7 - Integrating Activities), Project POCI-01-0145-FEDER-006984–Associate Laboratory LSRE-LCM (UID/EQU/50020/2013) and POCI-01-0145-FEDER-006939 (LEPABE – UID/EQU/00511/2013), funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia; UID/Multi/50016/2013-CBQF and Water JPI/0001/2013 STARE, and partially co-financed by QREN, ON2, FCT and FEDER through project AIProcMat@N2020 NORTE-01-0145-FEDER-000006, NORTE-07-0162-FEDER-000050 and NORTE-01-0145-FEDER-000005 (LEPABE-2-ECO-INNOVATION), supported by North Portugal Regional Operational Program (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the ERDF. NFFM, LMPM and AMTS acknowledge PD/BD/114318/2016, IF/01248/2014 and IF/01501/2013, respectively. The authors would like to acknowledge the financial support provided by COST-European Cooperation in Science and Technology, to the COST Action ES1403: New and emerging challenges and opportunities in wastewater reuse (NEREUS). Disclaimer: The content of this article is the authors' responsibility and neither COST nor any person acting on its behalf is responsible for the use, which might be made of the information contained in it.
Funding Information:
This work was financially supported by Project nº P1404290052 under the SFERA Program (EC/FP7 - Integrating Activities) , Project POCI-01-0145-FEDER-006984–Associate Laboratory LSRE-LCM ( UID/EQU/50020/2013 ) and POCI-01-0145-FEDER-006939 ( LEPABE – UID/EQU/00511/2013 ), funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia ; UID/Multi/50016/2013-CBQF and Water JPI/0001/2013 STARE , and partially co-financed by QREN , ON2 , FCT and FEDER through project AIProcMat@N2020 NORTE-01-0145-FEDER-000006 , NORTE-07-0162-FEDER-000050 and NORTE-01-0145-FEDER-000005 (LEPABE-2-ECO-INNOVATION), supported by North Portugal Regional Operational Program (NORTE 2020) , under the Portugal 2020 Partnership Agreement, through the ERDF . NFFM, LMPM and AMTS acknowledge PD/BD/114318/2016, IF/01248/2014 and IF/01501/2013, respectively. The authors would like to acknowledge the financial support provided by COST-European Cooperation in Science and Technology , to the COST Action ES1403: New and emerging challenges and opportunities in wastewater reuse (NEREUS) . Disclaimer: The content of this article is the authors' responsibility and neither COST nor any person acting on its behalf is responsible for the use, which might be made of the information contained in it.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/5/15
Y1 - 2018/5/15
N2 - Solar-driven advanced oxidation processes were studied in a pilot-scale photoreactor, as tertiary treatments of effluents from an urban wastewater treatment plant. Solar-H2O2, heterogeneous photocatalysis (with and/or without the addition of H2O2 and employing three different photocatalysts) and the photo-Fenton process were investigated. Chemical (sulfamethoxazole, carbamazepine, and diclofenac) and biological contaminants (faecal contamination indicators, their antibiotic resistant counterparts, 16S rRNA and antibiotic resistance genes), as well as the whole bacterial community, were characterized. Heterogeneous photocatalysis using TiO2-P25 and assisted with H2O2 (P25/H2O2) was the most efficient process on the degradation of the chemical organic micropollutants, attaining levels below the limits of quantification in less than 4 h of treatment (corresponding to QUV < 40 kJ L−1). This performance was followed by the same process without H2O2, using TiO2-P25 or a composite material based on graphene oxide and TiO2. Regarding the biological indicators, total faecal coliforms and enterococci and their antibiotic resistant (tetracycline and ciprofloxacin) counterparts were reduced to values close, or beneath, the detection limit (1 CFU 100 mL−1) for all treatments employing H2O2, even upon storage of the treated wastewater for 3-days. Moreover, P25/H2O2 and solar-H2O2 were the most efficient processes in the reduction of the abundance (gene copy number per volume of wastewater) of the analysed genes. However, this reduction was transient for 16S rRNA, intI1 and sul1 genes, since after 3-days storage of the treated wastewater their abundance increased to values close to pre-treatment levels. Similar behaviour was observed for the genes qnrS (using TiO2-P25), blaCTX-M and blaTEM (using TiO2-P25 and TiO2-P25/H2O2). Interestingly, higher proportions of sequence reads affiliated to the phylum Proteobacteria (Beta- and Gammaproteobacteria) were found after 3-days storage of treated wastewater than before its treatment. Members of the genera Pseudomonas, Rheinheimera and Methylotenera were among those with overgrowth.
AB - Solar-driven advanced oxidation processes were studied in a pilot-scale photoreactor, as tertiary treatments of effluents from an urban wastewater treatment plant. Solar-H2O2, heterogeneous photocatalysis (with and/or without the addition of H2O2 and employing three different photocatalysts) and the photo-Fenton process were investigated. Chemical (sulfamethoxazole, carbamazepine, and diclofenac) and biological contaminants (faecal contamination indicators, their antibiotic resistant counterparts, 16S rRNA and antibiotic resistance genes), as well as the whole bacterial community, were characterized. Heterogeneous photocatalysis using TiO2-P25 and assisted with H2O2 (P25/H2O2) was the most efficient process on the degradation of the chemical organic micropollutants, attaining levels below the limits of quantification in less than 4 h of treatment (corresponding to QUV < 40 kJ L−1). This performance was followed by the same process without H2O2, using TiO2-P25 or a composite material based on graphene oxide and TiO2. Regarding the biological indicators, total faecal coliforms and enterococci and their antibiotic resistant (tetracycline and ciprofloxacin) counterparts were reduced to values close, or beneath, the detection limit (1 CFU 100 mL−1) for all treatments employing H2O2, even upon storage of the treated wastewater for 3-days. Moreover, P25/H2O2 and solar-H2O2 were the most efficient processes in the reduction of the abundance (gene copy number per volume of wastewater) of the analysed genes. However, this reduction was transient for 16S rRNA, intI1 and sul1 genes, since after 3-days storage of the treated wastewater their abundance increased to values close to pre-treatment levels. Similar behaviour was observed for the genes qnrS (using TiO2-P25), blaCTX-M and blaTEM (using TiO2-P25 and TiO2-P25/H2O2). Interestingly, higher proportions of sequence reads affiliated to the phylum Proteobacteria (Beta- and Gammaproteobacteria) were found after 3-days storage of treated wastewater than before its treatment. Members of the genera Pseudomonas, Rheinheimera and Methylotenera were among those with overgrowth.
KW - Antibiotic resistance genes
KW - Bacterial community composition
KW - Faecal indicators
KW - Solar advanced oxidation processes
KW - Urban wastewater
UR - http://www.scopus.com/inward/record.url?scp=85042297132&partnerID=8YFLogxK
U2 - 10.1016/j.watres.2018.01.064
DO - 10.1016/j.watres.2018.01.064
M3 - Article
C2 - 29475109
AN - SCOPUS:85042297132
SN - 0043-1354
VL - 135
SP - 195
EP - 206
JO - Water Research
JF - Water Research
ER -