TY - JOUR
T1 - Sphingobium vermicomposti sp. nov., isolated from vermicompost
AU - Vaz-Moreira, Ivone
AU - Faria, Cátia
AU - Lopes, Ana R.
AU - Svensson, Liselott
AU - Falsen, Enevold
AU - Moore, Edward R.B.
AU - Ferreira, António C. Silva
AU - Nunes, Olga C.
AU - Manaia, Célia M.
PY - 2009/12/22
Y1 - 2009/12/22
N2 - Strain VC-230T was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15-37 °C and pH 6-8. On the basis of 16S rRNA gene sequence analysis, strain VC-230T was determined to belong to the family Sphingomonadaceae by its clustering with type strains of the genus Sphingobium, with Sphingobium chlorophenolicum ATCC 33790T (97.7 %) and Sphingobium herbicidovorans DSM 11019T (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C18:1ω7c/9t/12t, C16:1ω7c and C16:0 and the G+C content of the genomic DNA supported the affiliation of this organism to the genus Sphingobium. The phylogenetic, chemotaxonomic, phenotypic and DNA-DNA hybridization analyses verify that strain VC-230T represents a novel species, for which the name Sphingobium vermicomposti sp. nov. is proposed. The type strain is VC-230T (=CCUG 55809T =DSM 21299T).
AB - Strain VC-230T was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15-37 °C and pH 6-8. On the basis of 16S rRNA gene sequence analysis, strain VC-230T was determined to belong to the family Sphingomonadaceae by its clustering with type strains of the genus Sphingobium, with Sphingobium chlorophenolicum ATCC 33790T (97.7 %) and Sphingobium herbicidovorans DSM 11019T (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C18:1ω7c/9t/12t, C16:1ω7c and C16:0 and the G+C content of the genomic DNA supported the affiliation of this organism to the genus Sphingobium. The phylogenetic, chemotaxonomic, phenotypic and DNA-DNA hybridization analyses verify that strain VC-230T represents a novel species, for which the name Sphingobium vermicomposti sp. nov. is proposed. The type strain is VC-230T (=CCUG 55809T =DSM 21299T).
UR - http://www.scopus.com/inward/record.url?scp=72149129482&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.006163-0
DO - 10.1099/ijs.0.006163-0
M3 - Article
C2 - 19643879
AN - SCOPUS:72149129482
SN - 1466-5026
VL - 59
SP - 3145
EP - 3149
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 12
ER -