TY - JOUR
T1 - Starch-based films doped with porphyrinoid photosensitizers for active skin wound healing
AU - Lopes, Paloma
AU - Joaquinito, A. Sofia M.
AU - Ribeiro, Artur
AU - Moura, Nuno M.M.
AU - Gomes, Ana T.P.
AU - Guerreiro, Susana G.
AU - Faustino, M. Amparo F.
AU - Almeida, Adelaide
AU - Ferreira, Paula
AU - Coimbra, Manuel A.
AU - Neves, M. Graça P.M.S.
AU - Gonçalves, Idalina
N1 - Funding Information:
The authors thank to University of Aveiro and FCT/MCT for the financial support provided to CICECO (UIDB/50011/2020, UIDP/50011/2020, LA/P/0006/2020), LAQV-REQUIMTE (UIDB/50006/2020 and UIDP/50006/2020), CESAM (UIDP/50017/2020, UIDB/50017/2020, LA/P/0094/2020), CEB (UIDB/04469/2020), LABBELS (LA/P/0029/2020), and to projects PORP2PS (EXPL/QUI-QOR/0586/2021) and PREVINE (FCT-PTDC/ASP-PES/29576/2017), through national funds (OE) and where applicable co-financed by the FEDER - Operational Thematic Program for Competitiveness and Internationalization - COMPETE 2020, within the PT2020 Partnership Agreement. Thanks are also due to the Portuguese NMR and Mass Networks. FCT also funded ASMJ PhD grant (2021.06854.BD), Investigator FCT program (PF, IF/00300/2015), and the Scientific Employment Stimulus program (IG, CEECIND/00430/2017; AR, 2021.02803.CEECIND). NMMM thanks FCT for funding through program DL 57/2016 (CDL-CTTRI-048-88-ARH/2018). The authors also acknowledge to POTATOPLASTIC project (POCI-01-0247-FEDER-017938), financed by FEDER through POCI, to Isolago – Indústria de Plásticos, S. A. the project leader, and to A Saloinha, Lda. company for providing the starch-rich potato washing slurries.
Funding Information:
The authors thank to University of Aveiro and FCT / MCT for the financial support provided to CICECO ( UIDB/50011/2020 , UIDP/50011/2020 , LA/P/0006/2020 ), LAQV-REQUIMTE ( UIDB/50006/2020 and UIDP/50006/2020 ), CESAM ( UIDP/50017/2020 , UIDB/50017/2020 , LA/P/0094/2020 ), CEB ( UIDB/04469/2020 ), LABBELS ( LA/P/0029/2020 ), and to projects PORP2PS ( EXPL/QUI-QOR/0586/2021 ) and PREVINE ( FCT-PTDC/ASP-PES/29576/2017 ), through national funds ( OE ) and where applicable co-financed by the FEDER - Operational Thematic Program for Competitiveness and Internationalization - COMPETE 2020, within the PT2020 Partnership Agreement. Thanks are also due to the Portuguese NMR and Mass Networks. FCT also funded ASMJ PhD grant ( 2021.06854.BD ), Investigator FCT program (PF, IF/00300/2015 ), and the Scientific Employment Stimulus program (IG, CEECIND/00430/2017 ; AR, 2021.02803.CEECIND ). NMMM thanks FCT for funding through program DL 57/2016 ( CDL-CTTRI-048-88-ARH/2018 ). The authors also acknowledge to POTATOPLASTIC project ( POCI-01-0247-FEDER-017938 ), financed by FEDER through POCI, to Isolago – Indústria de Plásticos, S. A., the project leader, and to A Saloinha, Lda. company for providing the starch-rich potato washing slurries.
Publisher Copyright:
© 2023 The Authors
PY - 2023/8/1
Y1 - 2023/8/1
N2 - Starch is a biodegradable and biocompatible carbohydrate that, when combined with bioactive molecules, can be processed as biomimetic platforms with enhanced performance, allowing its use as active wound dressing materials. Porphyrinoid photosensitizers can tune the physicochemical/functional profile of biomacromolecules, allowing their use in anti-infective strategies. In this work, the feasibility of using the cationic 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetraiodide (TMPyP) to enhance the physicochemical, mechanical, antimicrobial performance, and wound healing ability of casted starch-based films was studied. TMPyP conferred a reddish coloration to the films, maintaining their pristine transparency. It increased by 87 % the films hydrophobicity and, depending on the TMPyP used, conferred mobility to the starch polymeric chains. Starch/TMPyP-based films effectively photoinactivated Escherichia coli (>99.99 %) and favored the wound healing process, even in the absence of light. Therefore, the incorporation of TMPyP into starch-based formulations revealed to be a promising strategy to tune the films compaction degree while giving rise to water tolerant and photosensitive biomaterials that can act as multitarget antimicrobial medical dressings and glycocarriers of active compounds relevant for effective skin wound healing.
AB - Starch is a biodegradable and biocompatible carbohydrate that, when combined with bioactive molecules, can be processed as biomimetic platforms with enhanced performance, allowing its use as active wound dressing materials. Porphyrinoid photosensitizers can tune the physicochemical/functional profile of biomacromolecules, allowing their use in anti-infective strategies. In this work, the feasibility of using the cationic 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetraiodide (TMPyP) to enhance the physicochemical, mechanical, antimicrobial performance, and wound healing ability of casted starch-based films was studied. TMPyP conferred a reddish coloration to the films, maintaining their pristine transparency. It increased by 87 % the films hydrophobicity and, depending on the TMPyP used, conferred mobility to the starch polymeric chains. Starch/TMPyP-based films effectively photoinactivated Escherichia coli (>99.99 %) and favored the wound healing process, even in the absence of light. Therefore, the incorporation of TMPyP into starch-based formulations revealed to be a promising strategy to tune the films compaction degree while giving rise to water tolerant and photosensitive biomaterials that can act as multitarget antimicrobial medical dressings and glycocarriers of active compounds relevant for effective skin wound healing.
KW - Antimicrobial
KW - Cellular migration
KW - Elasticity
KW - Hydrophobicity
KW - Polysaccharides
KW - Porphyrins
UR - http://www.scopus.com/inward/record.url?scp=85152480045&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2023.120894
DO - 10.1016/j.carbpol.2023.120894
M3 - Article
C2 - 37182978
AN - SCOPUS:85152480045
SN - 0144-8617
VL - 313
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 120894
ER -