TY - JOUR
T1 - Tepidiphilus margaritifer gen. nov., sp nov., isolated from a thermophilic aerobic digester
AU - Manaia, Célia M.
AU - Nogales, Balbina
AU - Nunes, Olga C.
PY - 2003/9
Y1 - 2003/9
N2 - A moderately thermophilic bacterium is described, strain N2-214T , that was isolated from an enrichment culture, growing on caprolactone, obtained from a sample from a water-treatment sludge aerobic digester operating at temperatures around 60 6C. The organism was aerobic, Gram-negative, oxidase- and catalase-positive, with a polar flagellum, and capable of growth at temperatures as high as 61 6C. The major fatty acids of strain N2-214T were C16 : 0, C18 : 1 and cyclo-C19 : 0. The phylogenetic relationships of the strain, derived from 16S rRNA gene sequence comparisons, demonstrated it to be a member of the b-subclass of the Proteobacteria. The highest 16S rDNA sequence similarity of isolate N2-214T was to Azoarcus buckelii (91?9 %), Thauera aromatica (92 %) and Hydrogenophilus thermoluteolus (92?7 %). On the basis of phylogenetic analyses and physiological and chemotaxonomic characteristics, it is proposed that isolate N2-214T (=DSM 15129T =LMG 21637T ) represents a new genus and species, Tepidiphilus margaritifer gen. nov., sp. nov.
AB - A moderately thermophilic bacterium is described, strain N2-214T , that was isolated from an enrichment culture, growing on caprolactone, obtained from a sample from a water-treatment sludge aerobic digester operating at temperatures around 60 6C. The organism was aerobic, Gram-negative, oxidase- and catalase-positive, with a polar flagellum, and capable of growth at temperatures as high as 61 6C. The major fatty acids of strain N2-214T were C16 : 0, C18 : 1 and cyclo-C19 : 0. The phylogenetic relationships of the strain, derived from 16S rRNA gene sequence comparisons, demonstrated it to be a member of the b-subclass of the Proteobacteria. The highest 16S rDNA sequence similarity of isolate N2-214T was to Azoarcus buckelii (91?9 %), Thauera aromatica (92 %) and Hydrogenophilus thermoluteolus (92?7 %). On the basis of phylogenetic analyses and physiological and chemotaxonomic characteristics, it is proposed that isolate N2-214T (=DSM 15129T =LMG 21637T ) represents a new genus and species, Tepidiphilus margaritifer gen. nov., sp. nov.
UR - http://www.scopus.com/inward/record.url?scp=0141502479&partnerID=8YFLogxK
U2 - 10.1099/IJS.0.02538-0
DO - 10.1099/IJS.0.02538-0
M3 - Article
SN - 1466-5026
VL - 53
SP - 1405
EP - 1410
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
ER -