TY - JOUR
T1 - The acetate uptake transporter family motif “NPAPLGL(M/S)” is essential for substrate uptake
AU - Ribas, David
AU - Soares-Silva, Isabel
AU - Vieira, Daniel
AU - Sousa-Silva, Maria
AU - Sá-Pessoa, Joana
AU - Azevedo-Silva, João
AU - Viegas, Sandra Cristina
AU - Arraiano, Cecília Maria
AU - Diallinas, George
AU - Paiva, Sandra
AU - Soares, Pedro
AU - Casal, Margarida
N1 - Funding Information:
This work was supported by the strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) and the project PTDC/BIAMIC/5184/2014 funded by national funds through the Fundação para a Ciência e Tecnologia (FCT) I.P. and by the European Regional Development Fund (ERDF) through the COMPETE 2020 - Programa Operacional Competitividade e Internacionalização (POCI); by the project EcoAgriFood: Innovative green products and processes to promote AgriFood BioEconomy (operação NORTE-01-0145-FEDER-000009), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Work at ITQB NOVA was financially supported by Project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI). DR acknowledges FCT for the SFRH/BD/96166/2013 PhD grant. MSS acknowledges the Norte2020 for the UMINHO/BD/25/2016 PhD grant with the reference NORTE-08-5369-FSE-000060.
Funding Information:
This work was supported by the strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) and the project PTDC/BIAMIC/5184/2014 funded by national funds through the Fundação para a Ciência e Tecnologia (FCT) I.P. and by the European Regional Development Fund (ERDF) through the COMPETE 2020 - Programa Operacional Competitividade e Internacionalização (POCI); by the project EcoAgriFood: Innovative green products and processes to promote AgriFood BioEconomy (operação NORTE-01-0145-FEDER-000009), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Work at ITQB NOVA was financially supported by Project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI). DR acknowledges FCT for the SFRH/BD/96166/2013 PhD grant. MSS acknowledges the Norte2020 for the UMINHO/BD/25/2016 PhD grant with the reference NORTE-08-5369-FSE-000060.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2019/1
Y1 - 2019/1
N2 - Organic acids are recognized as one of the most prevalent compounds in ecosystems, thus the transport and assimilation of these molecules represent an adaptive advantage for organisms. The AceTr family members are associated with the active transport of organic acids, namely acetate and succinate. The phylogenetic analysis shows this family is dispersed in the tree of life. However, in eukaryotes, it is almost limited to microbes, though reaching a prevalence close to 100% in fungi, with an essential role in spore development. Aiming at deepening the knowledge in this family, we studied the acetate permease AceP from Methanosarcina acetivorans, as the first functionally characterized archaeal member of this family. Furthermore, we demonstrate that the yeast Gpr1 from Yarrowia lipolytica is an acetate permease, whereas the Ady2 closest homologue in Saccharomyces cerevisiae, Fun34, has no role in acetate uptake. In this work, we describe the functional role of the AceTr conserved motif NPAPLGL(M/S). We further unveiled the role of the amino acid residues R122 and Q125 of SatP as essential for protein activity.
AB - Organic acids are recognized as one of the most prevalent compounds in ecosystems, thus the transport and assimilation of these molecules represent an adaptive advantage for organisms. The AceTr family members are associated with the active transport of organic acids, namely acetate and succinate. The phylogenetic analysis shows this family is dispersed in the tree of life. However, in eukaryotes, it is almost limited to microbes, though reaching a prevalence close to 100% in fungi, with an essential role in spore development. Aiming at deepening the knowledge in this family, we studied the acetate permease AceP from Methanosarcina acetivorans, as the first functionally characterized archaeal member of this family. Furthermore, we demonstrate that the yeast Gpr1 from Yarrowia lipolytica is an acetate permease, whereas the Ady2 closest homologue in Saccharomyces cerevisiae, Fun34, has no role in acetate uptake. In this work, we describe the functional role of the AceTr conserved motif NPAPLGL(M/S). We further unveiled the role of the amino acid residues R122 and Q125 of SatP as essential for protein activity.
KW - Acetate transport
KW - AceTr family phylogeny
KW - Carboxylate transporters
KW - Cell membrane
KW - Succinate transport
UR - http://www.scopus.com/inward/record.url?scp=85055317896&partnerID=8YFLogxK
U2 - 10.1016/j.fgb.2018.10.001
DO - 10.1016/j.fgb.2018.10.001
M3 - Article
C2 - 30339831
AN - SCOPUS:85055317896
SN - 1087-1845
VL - 122
SP - 1
EP - 10
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
ER -