TY - JOUR
T1 - The evolutionary portrait of metazoan NAD salvage
AU - Carneiro, João
AU - Duarte-Pereira, Sara
AU - Azevedo, Luísa
AU - Castro, L. Filipe C.
AU - Aguiar, Paulo
AU - Moreira, Irina S.
AU - Amorim, António
AU - Silva, Raquel M.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/5/28
Y1 - 2013/5/28
N2 - Nicotinamide Adenine Dinucleotide (NAD) levels are essential for cellular homeostasis and survival. Main sources of intracellular NAD are the salvage pathways from nicotinamide, where Nicotinamide phosphoribosyltransferases (NAMPTs) and Nicotinamidases (PNCs) have a key role. NAMPTs and PNCs are important in aging, infection and disease conditions such as diabetes and cancer. These enzymes have been considered redundant since either one or the other exists in each individual genome. The co-occurrence of NAMPT and PNC was only recently detected in invertebrates though no structural or functional characterization exists for them. Here, using expression and evolutionary analysis combined with homology modeling and protein-ligand docking, we show that both genes are expressed simultaneously in key species of major invertebrate branches and emphasize sequence and structural conservation patterns in metazoan NAMPT and PNC homologues. The results anticipate that NAMPTs and PNCs are simultaneously active, raising the possibility that NAD salvage pathways are not redundant as both are maintained to fulfill the requirement for NAD production in some species.
AB - Nicotinamide Adenine Dinucleotide (NAD) levels are essential for cellular homeostasis and survival. Main sources of intracellular NAD are the salvage pathways from nicotinamide, where Nicotinamide phosphoribosyltransferases (NAMPTs) and Nicotinamidases (PNCs) have a key role. NAMPTs and PNCs are important in aging, infection and disease conditions such as diabetes and cancer. These enzymes have been considered redundant since either one or the other exists in each individual genome. The co-occurrence of NAMPT and PNC was only recently detected in invertebrates though no structural or functional characterization exists for them. Here, using expression and evolutionary analysis combined with homology modeling and protein-ligand docking, we show that both genes are expressed simultaneously in key species of major invertebrate branches and emphasize sequence and structural conservation patterns in metazoan NAMPT and PNC homologues. The results anticipate that NAMPTs and PNCs are simultaneously active, raising the possibility that NAD salvage pathways are not redundant as both are maintained to fulfill the requirement for NAD production in some species.
UR - http://www.scopus.com/inward/record.url?scp=84878391235&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0064674
DO - 10.1371/journal.pone.0064674
M3 - Article
C2 - 23724078
AN - SCOPUS:84878391235
SN - 1932-6203
VL - 8
JO - PLoS one
JF - PLoS one
IS - 5
M1 - e64674
ER -