TY - JOUR
T1 - The impact of antibiotic exposure in water and zebrafish gut microbiomes
T2 - a 16S rRNA gene-based metagenomic analysis
AU - Almeida, Ana Rita
AU - Alves, Marta
AU - Domingues, Inês
AU - Henriques, Isabel
N1 - Funding Information:
This work was supported by National Funds from FCT - Fundação para a Ciência e a Tecnologia - through the project GReAT - PTDC/BTA-BTA/29970/2017 ( POCI-01-0145-FEDER-029970 ). Thanks are due for the financial support to CESAM ( UID/AMB/50017/2019 ), to FCT/MCTES through national funds. Support was also given by FCT to Ana Rita Almeida by PhD grant ( PD/BD/127811/2016 ). Inês Domingues is funded by national funds, through FCT – Fundação para a Ciência e a Tecnologia , I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.
Funding Information:
This work was supported by National Funds from FCT - Funda??o para a Ci?ncia e a Tecnologia - through the project GReAT - PTDC/BTA-BTA/29970/2017 (POCI-01-0145-FEDER-029970). Thanks are due for the financial support to CESAM (UID/AMB/50017/2019), to FCT/MCTES through national funds. Support was also given by FCT to Ana Rita Almeida by PhD grant (PD/BD/127811/2016). In?s Domingues is funded by national funds, through FCT ? Funda??o para a Ci?ncia e a Tecnologia, I.P. in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/12/30
Y1 - 2019/12/30
N2 - In order to supply human demand for food, the aquaculture industry has been growing fast in the last years, being fish usually cultivated in overcrowded conditions. Hence, to prevent the rapidly disease spreading, antibiotics may be applied to both sick and healthy animals. Due to its broad spectrum, oxytetracycline (OTC) is one of the most used antibiotics in food-production. Yet, although useful to prevent infections, antibiotics may reshape aquatic animals' microbiome, disturbing hosts’ welfare. However, the impact of this exposure to the organism microbiome and its surrounding environment is poorly understood. Then, the objective of this study was to analyze in detail the long-term effect of OTC in both zebrafish gut and water microbiomes. Zebrafish adults were exposed, via water, for two months to three concentrations of OTC (0, 10 and 10000 μg/L). Total DNA was extracted from gut and water samples and the V3–V4 region of the bacterial 16 S rRNA gene was sequenced using Illumina technology. Results of alpha and beta-diversity analyses revealed that long-term exposure to OTC impacted both zebrafish gut and water microbiomes. In water samples, effects were observed even at the lowest (10 μg/L) OTC concentration tested resulting in an increase in Deltaproteobacteria, namely the Myxococcales and Bdellovibrionales orders. On the other hand, effects on zebrafish gut were only observed at the highest concentration with the selection of Alphaproteobacteria and Actinobacteria classes. Although these classes are common in fish gut, the increase of Actinobacteria may represent a health problem since some genera like Gordonia are linked to some human infection disease. Nevertheless, in both gut and water, it was observed a decrease in Gamaproteobacteria, probably due to OTC mode of action. In silico functional metagenomic analysis revealed that OTC exposure selected general detoxification mechanisms. In addition, the abundance of functional genes involved in Quorum Sensing (QS) increased under OTC exposure suggesting that QS may help bacteria to survive OTC stress. Thus, future studies should consider post-exposure scenarios for a deeper analysis of the water and zebrafish gut resistome, since bacteria may react differently after exposure ceased.
AB - In order to supply human demand for food, the aquaculture industry has been growing fast in the last years, being fish usually cultivated in overcrowded conditions. Hence, to prevent the rapidly disease spreading, antibiotics may be applied to both sick and healthy animals. Due to its broad spectrum, oxytetracycline (OTC) is one of the most used antibiotics in food-production. Yet, although useful to prevent infections, antibiotics may reshape aquatic animals' microbiome, disturbing hosts’ welfare. However, the impact of this exposure to the organism microbiome and its surrounding environment is poorly understood. Then, the objective of this study was to analyze in detail the long-term effect of OTC in both zebrafish gut and water microbiomes. Zebrafish adults were exposed, via water, for two months to three concentrations of OTC (0, 10 and 10000 μg/L). Total DNA was extracted from gut and water samples and the V3–V4 region of the bacterial 16 S rRNA gene was sequenced using Illumina technology. Results of alpha and beta-diversity analyses revealed that long-term exposure to OTC impacted both zebrafish gut and water microbiomes. In water samples, effects were observed even at the lowest (10 μg/L) OTC concentration tested resulting in an increase in Deltaproteobacteria, namely the Myxococcales and Bdellovibrionales orders. On the other hand, effects on zebrafish gut were only observed at the highest concentration with the selection of Alphaproteobacteria and Actinobacteria classes. Although these classes are common in fish gut, the increase of Actinobacteria may represent a health problem since some genera like Gordonia are linked to some human infection disease. Nevertheless, in both gut and water, it was observed a decrease in Gamaproteobacteria, probably due to OTC mode of action. In silico functional metagenomic analysis revealed that OTC exposure selected general detoxification mechanisms. In addition, the abundance of functional genes involved in Quorum Sensing (QS) increased under OTC exposure suggesting that QS may help bacteria to survive OTC stress. Thus, future studies should consider post-exposure scenarios for a deeper analysis of the water and zebrafish gut resistome, since bacteria may react differently after exposure ceased.
KW - Danio rerio
KW - Microbiome
KW - OTU
KW - Piphillin
KW - Tetracycline
UR - http://www.scopus.com/inward/record.url?scp=85073241872&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2019.109771
DO - 10.1016/j.ecoenv.2019.109771
M3 - Article
C2 - 31629904
AN - SCOPUS:85073241872
SN - 0147-6513
VL - 186
SP - 1
EP - 10
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
M1 - 109771
ER -
