TY - JOUR
T1 - The influence of rhBMP-7 associated with nanometric hydroxyapatite coatings titanium implant on the osseointegration
T2 - a pre-clinical study
AU - Bonato, Rafael Silva
AU - Fernandes, Gustavo Vicentis de Oliveira
AU - Calasans-Maia, Monica Diuana
AU - Mello, Alexandre
AU - Rossi, Alexandre Malta
AU - Carreira, Ana Claudia Oliveira
AU - Sogayar, Mari Cleide
AU - Granjeiro, José Mauro
N1 - Funding Information:
This work was funded by grants from the Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ), No. E-26/010.002989/2014, E-26/010.000210/2019, FAPESP (São Paulo Research Foundation)—Thematic Grant No. 2016/05311-2, the Brazilian National Council for Science and Technology (CNPq) research grants Nos. 465656/2014-5 to INCT-Regenera and 401430/2013-8 and CAPES (Coordination for Higher Education and Training) PROBITEC grant No. 23038.004161/2012-9, FINEP-Studies and Projects Brazilian Financing Agency No. 01.08.0622.00), and BNDES-Brazilian National Bank for Social and Economic Development No. 09.2.1066.1.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/9/26
Y1 - 2022/9/26
N2 - Background: Bioceramic nanometer coatings have been regarded as potential substitutes for plasma-sprayed hydroxyapatite coatings, and the association with bone morphogenetic protein (BMP) is an attempt to achieve faster osseointegration to hasten oral rehabilitation. Objective: This study aimed to investigate the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the osseointegration of titanium implants coated with a thin film surface of hydroxyapatite (HA). Methods: Two implants (n = 24) were placed in each white New Zealand rabbits’ femur (n = 6). Implants were placed in the right femur after standard instrumentation (A and B) and in the left femur after an over-instrumentation (C and D), preventing bone-implant contact. The distal implants were installed associated with rhBMP-7 (groups B [regular instrumentation] and D [over-instrumentation]) and, also, in the absence of without BMP (control groups A [regular instrumentation] and C [over-instrumentation]). After 4 weeks, the animals were euthanized. The bone blocks containing the implants were embedded in methyl methacrylate and sectioned parallel to the long axis of the implant, which were analyzed by image segmentation. The data were analyzed using a nonparametric statistical method. Results: We observed that Group A had a mean bone formation of 35.6% compared to Group B, which had 48.6% (p > 0.05). Moreover, this group showed 28.3% of connective tissue compared to Group A, with 39.3%. In the over-instrumented groups, rhBMP-7 (Group D) showed an enhanced and significant increase in bone formation when compared with the group without rhBMP-7 (Group C). Conclusion: We concluded that the association of rhBMP-7 to thin nanostructure HA-coated implants promoted greater new bone area than the same implants in the absence of rhBMP-7, mainly in cases of over-instrumented implant sites.
AB - Background: Bioceramic nanometer coatings have been regarded as potential substitutes for plasma-sprayed hydroxyapatite coatings, and the association with bone morphogenetic protein (BMP) is an attempt to achieve faster osseointegration to hasten oral rehabilitation. Objective: This study aimed to investigate the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the osseointegration of titanium implants coated with a thin film surface of hydroxyapatite (HA). Methods: Two implants (n = 24) were placed in each white New Zealand rabbits’ femur (n = 6). Implants were placed in the right femur after standard instrumentation (A and B) and in the left femur after an over-instrumentation (C and D), preventing bone-implant contact. The distal implants were installed associated with rhBMP-7 (groups B [regular instrumentation] and D [over-instrumentation]) and, also, in the absence of without BMP (control groups A [regular instrumentation] and C [over-instrumentation]). After 4 weeks, the animals were euthanized. The bone blocks containing the implants were embedded in methyl methacrylate and sectioned parallel to the long axis of the implant, which were analyzed by image segmentation. The data were analyzed using a nonparametric statistical method. Results: We observed that Group A had a mean bone formation of 35.6% compared to Group B, which had 48.6% (p > 0.05). Moreover, this group showed 28.3% of connective tissue compared to Group A, with 39.3%. In the over-instrumented groups, rhBMP-7 (Group D) showed an enhanced and significant increase in bone formation when compared with the group without rhBMP-7 (Group C). Conclusion: We concluded that the association of rhBMP-7 to thin nanostructure HA-coated implants promoted greater new bone area than the same implants in the absence of rhBMP-7, mainly in cases of over-instrumented implant sites.
KW - Bone morphogenetic proteins
KW - Coated materials
KW - Dental implants
KW - Pre-clinical study
UR - http://www.scopus.com/inward/record.url?scp=85139855897&partnerID=8YFLogxK
U2 - 10.3390/polym14194030
DO - 10.3390/polym14194030
M3 - Article
C2 - 36235978
SN - 2073-4360
VL - 14
JO - Polymers
JF - Polymers
IS - 19
M1 - 4030
ER -