The isolation of RNA from raspberry (Rubus idaeus) fruit

Chris S. Jones, Pietro P.M. Iannetta, Mary Woodhead, Howard V. Davies, Ronnie J. McNicol, Mark A. Taylor*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Previous attempts to extract high-quality, total RNA from raspberry (Rubus idaeus) fruits using published protocols have proven to be unsuccessful. Even the use of protocols developed for the extraction of RNA from other fruit tissue has resulted in low yields (1) or the isolation of degraded RNA (2). Here, we report on the development of a quick and simple method of extracting total RNA from raspberry fruit. Using this method, high yields of good quality, undegraded RNA were obtained from fruit at all stages of ripening. The RNA is of sufficient quality for northern analysis and cDNA library construction.

Original languageEnglish
Pages (from-to)219-221
Number of pages3
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Issue number3
Publication statusPublished - Dec 1997
Externally publishedYes


  • Fruit tissue
  • Raspberry
  • RNA extraction
  • Rubus idaeus


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