The isolation of RNA from raspberry (Rubus idaeus) fruit

Chris S. Jones; Pietro P.M. Iannetta; Mary Woodhead; Howard V. Davies; Ronnie J. McNicol; Mark A. Taylor

doi:10.1007/BF02760775

The isolation of RNA from raspberry (Rubus idaeus) fruit

Chris S. Jones, Pietro P.M. Iannetta, Mary Woodhead, Howard V. Davies, Ronnie J. McNicol, Mark A. Taylor^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Previous attempts to extract high-quality, total RNA from raspberry (Rubus idaeus) fruits using published protocols have proven to be unsuccessful. Even the use of protocols developed for the extraction of RNA from other fruit tissue has resulted in low yields (1) or the isolation of degraded RNA (2). Here, we report on the development of a quick and simple method of extracting total RNA from raspberry fruit. Using this method, high yields of good quality, undegraded RNA were obtained from fruit at all stages of ripening. The RNA is of sufficient quality for northern analysis and cDNA library construction.

Original language	English
Pages (from-to)	219-221
Number of pages	3
Journal	Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume	8
Issue number	3
DOIs	https://doi.org/10.1007/BF02760775
Publication status	Published - Dec 1997
Externally published	Yes

Keywords

Fruit tissue
Raspberry
RNA extraction
Rubus idaeus

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10.1007/BF02760775

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title = "The isolation of RNA from raspberry (Rubus idaeus) fruit",

abstract = "Previous attempts to extract high-quality, total RNA from raspberry (Rubus idaeus) fruits using published protocols have proven to be unsuccessful. Even the use of protocols developed for the extraction of RNA from other fruit tissue has resulted in low yields (1) or the isolation of degraded RNA (2). Here, we report on the development of a quick and simple method of extracting total RNA from raspberry fruit. Using this method, high yields of good quality, undegraded RNA were obtained from fruit at all stages of ripening. The RNA is of sufficient quality for northern analysis and cDNA library construction.",

keywords = "Fruit tissue, Raspberry, RNA extraction, Rubus idaeus",

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AU - Jones, Chris S.

AU - Iannetta, Pietro P.M.

AU - Woodhead, Mary

AU - Davies, Howard V.

AU - McNicol, Ronnie J.

AU - Taylor, Mark A.

PY - 1997/12

Y1 - 1997/12

N2 - Previous attempts to extract high-quality, total RNA from raspberry (Rubus idaeus) fruits using published protocols have proven to be unsuccessful. Even the use of protocols developed for the extraction of RNA from other fruit tissue has resulted in low yields (1) or the isolation of degraded RNA (2). Here, we report on the development of a quick and simple method of extracting total RNA from raspberry fruit. Using this method, high yields of good quality, undegraded RNA were obtained from fruit at all stages of ripening. The RNA is of sufficient quality for northern analysis and cDNA library construction.

AB - Previous attempts to extract high-quality, total RNA from raspberry (Rubus idaeus) fruits using published protocols have proven to be unsuccessful. Even the use of protocols developed for the extraction of RNA from other fruit tissue has resulted in low yields (1) or the isolation of degraded RNA (2). Here, we report on the development of a quick and simple method of extracting total RNA from raspberry fruit. Using this method, high yields of good quality, undegraded RNA were obtained from fruit at all stages of ripening. The RNA is of sufficient quality for northern analysis and cDNA library construction.

KW - Fruit tissue

KW - Raspberry

KW - RNA extraction

KW - Rubus idaeus

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ER -

The isolation of RNA from raspberry (Rubus idaeus) fruit

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Keywords

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