TY - JOUR
T1 - The magic cocktail
T2 - ampicillin and biosynthesized gold nanoparticles synergism against staphylococcus aureus
AU - Oliveira, Marco
AU - Soares, Silvia
AU - Sá, Sara
AU - Gestoso, Álvaro
AU - Correa-Duarte, Miguel
AU - Baylina, Pilar
AU - Fernandes, Rúben
AU - Pereira, Carla F.
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/10
Y1 - 2023/10
N2 - Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 °C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500–600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.
AB - Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 °C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500–600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.
KW - Ampicillin
KW - Antibiotic resistance
KW - Biosynthetic gold nanoparticles
KW - Synergism
UR - http://www.scopus.com/inward/record.url?scp=85174172604&partnerID=8YFLogxK
U2 - 10.3390/app131910934
DO - 10.3390/app131910934
M3 - Article
AN - SCOPUS:85174172604
SN - 2076-3417
VL - 13
JO - Applied Sciences (Switzerland)
JF - Applied Sciences (Switzerland)
IS - 19
M1 - 10934
ER -
